Refactoring Ehrlich Pathway for High-Yield 2-Phenylethanol Production in Yarrowia lipolytica
… strain produced 2-PE over 1200 mg/L. Then the top-performed strains were further validated
… ylDGA2 deletion on lipid production, we measured the lipid titer in strains po1fk4P5, po1fk5P…
… ylDGA2 deletion on lipid production, we measured the lipid titer in strains po1fk4P5, po1fk5P…
A VersaTile-driven platform for rapid hit-to-lead development of engineered lysins
H Gerstmans, D Grimon, D Gutiérrez, C Lood… - Science …, 2020 - science.org
… strains, with more than half of them inhibiting all strains. … By extension, VersaTile can be used
to create DNA libraries that … the EMBOSS needle tool to verify both the order and the type of …
to create DNA libraries that … the EMBOSS needle tool to verify both the order and the type of …
Spatiotemporal developmental trajectories in the Arabidopsis root revealed using high-throughput single-cell RNA sequencing
… Following library production and sequencing, transcripts from individual cells can be
identified from the bead-derived barcode and individual transcripts accounted for using unique …
identified from the bead-derived barcode and individual transcripts accounted for using unique …
… ecosystem-specific reference databases with species-level resolution by high-throughput full-length 16S rRNA gene sequencing and automated taxonomy …
… and an analysis of 7,816 sequences previously obtained from the eight-strain ZymoBiomics
microbial community DNA standard (26) (see Text S1 in the supplemental material). …
microbial community DNA standard (26) (see Text S1 in the supplemental material). …
Genome engineering of E. coli for improved styrene production
… enable high throughput and scalable polystyrene production. … the FDC1 RBS library into the
host strain, and we observed a … After reconstruction and genomic verification of this edit, we …
host strain, and we observed a … After reconstruction and genomic verification of this edit, we …
[HTML][HTML] Investigation of gut microbiome changes in type 1 diabetic mellitus rats based on high-throughput sequencing
Q Ma, Y Li, J Wang, P Li, Y Duan, H Dai, Y An… - Biomedicine & …, 2020 - Elsevier
… DNA were extracted, 16S rRNA microbial gene clone library were constructed, and lastly
high-throughput sequencing … protect intestinal barrier function by producing short-chain fatty …
high-throughput sequencing … protect intestinal barrier function by producing short-chain fatty …
Ultra-high throughput mapping of genetic design space
… development of bioproduction strains 61 and multigenic … DNA sources and cloned into
kanamycin resistant sub-part entry vectors using BpiI (Thermofisher) (level 0). Sequence-verified …
kanamycin resistant sub-part entry vectors using BpiI (Thermofisher) (level 0). Sequence-verified …
High-efficiency nuclear transformation of the microalgae Nannochloropsis oceanica using Tn5 Transposome for the generation of altered lipid accumulation …
H Osorio, C Jara, K Fuenzalida, E Rey-Jurado… - Biotechnology for …, 2019 - Springer
… high-efficiency method for the generation of random mutant strains at the level of genomic
DNA … To verify that we obtained mutant clones of the lipid accumulation populations selected, …
DNA … To verify that we obtained mutant clones of the lipid accumulation populations selected, …
High-throughput sequencing approach to characterize dynamic changes of the fungal and bacterial communities during the production of sufu, a traditional Chinese …
D Xu, P Wang, X Zhang, J Zhang, Y Sun, L Gao… - Food …, 2020 - Elsevier
… DNA extraction by GMO food DNA Extraction Kit (Tiangen, Beijing, China) following the
manufacturer's protocol. The total DNA concentration and quality were checked … specific strains of …
manufacturer's protocol. The total DNA concentration and quality were checked … specific strains of …
Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation
… been validated in both modular and high-throughput settings. … as DNA library construction
for next-generation sequencing [4]. … Plasmid extraction from cultured Escherichia coli strains is …
for next-generation sequencing [4]. … Plasmid extraction from cultured Escherichia coli strains is …