Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters
Y Ahmed, Y Rebets, MR Estévez, J Zapp… - Microbial cell …, 2020 - Springer
… The utility of the developed strains was validated by expressing four secondary … genomic
library in the modified S. lividans ΔYA9 and S. albus Del14 strains resulted in the production of …
library in the modified S. lividans ΔYA9 and S. albus Del14 strains resulted in the production of …
Discovery and characterization of Cas9 inhibitors disseminated across seven bacterial phyla
… We identified 11 DNA fragments from soil, animal, and human metagenomes that … in our
selection strain. Further in vivo and in vitro characterization of a subset of these hits validated the …
selection strain. Further in vivo and in vitro characterization of a subset of these hits validated the …
Diagnosis of plant diseases using the Nanopore sequencing platform
L Chalupowicz, A Dombrovsky, V Gaba… - Plant …, 2019 - Wiley Online Library
… of samples with unidentified disease agents were validated by … Pathogenic strains used
in this study included bacteria: … above for 1D Genomic DNA library preparation. For each …
in this study included bacteria: … above for 1D Genomic DNA library preparation. For each …
A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing
… , multiomics, high throughput DNA sequencing and synthesis, … engineering methods to
speed up the strain development in a … The genomic library construction and illumina paired-end …
speed up the strain development in a … The genomic library construction and illumina paired-end …
A flow cytometry‐based assay for high‐throughput detection and quantification of neutrophil extracellular traps in mixed cell populations
O Zharkova, SH Tay, HY Lee, T Shubhita… - Cytometry Part …, 2019 - Wiley Online Library
… in B6 and the mild autoimmune prone strain, Sle1. (F) Sle123 … In this article, we detailed and
validated a novel flow … against PMN cell-surface markers with DNA dyes 50. NETs are very …
validated a novel flow … against PMN cell-surface markers with DNA dyes 50. NETs are very …
DNA targeting and interference by a bacterial Argonaute nuclease
A Kuzmenko, A Oguienko, D Esyunina, D Yudin… - Nature, 2020 - nature.com
… were selected and verified by PCR and sequencing. This … = 1.0; for strains with plasmid-encoded
CbAgo DNA was extracted … Preparation of genomic DNA libraries was carried out using …
CbAgo DNA was extracted … Preparation of genomic DNA libraries was carried out using …
River Ganges water as reservoir of microbes with antibiotic and metal ion resistance genes: High throughput metagenomic approach
… has led different resistant pathogenic strains to evolve. The use of … The quality and quantity
was checked on ND 2000 Nanodrop … TruSeq Nano DNA library preparation kit. The quality of …
was checked on ND 2000 Nanodrop … TruSeq Nano DNA library preparation kit. The quality of …
[HTML][HTML] Engineering phage host-range and suppressing bacterial resistance through phage tail fiber mutagenesis
… In addition, phages are selective for particular bacterial strains, as opposed to conventional
chemical antibiotics, most of which exhibit broad-spectrum activity, which contributes to …
chemical antibiotics, most of which exhibit broad-spectrum activity, which contributes to …
Application of combinatorial optimization strategies in synthetic biology
G Naseri, MAG Koffas - Nature communications, 2020 - nature.com
… of microbial strains in a library that produce the highest level … to time-consuming metabolite
screening techniques 39,40 . To … in vitro method to smartly generate DNA library construction …
screening techniques 39,40 . To … in vitro method to smartly generate DNA library construction …
High-throughput screening and identification of potent broad-spectrum inhibitors of coronaviruses
L Shen, J Niu, C Wang, B Huang, W Wang… - Journal of …, 2019 - Am Soc Microbiol
… We screened 56 hits from the HTS data and validated 36 compounds in vitro … strain (HCoV-OC43)
expressing Renilla luciferase (Rluc; rOC43-ns2Del-Rluc) facilitates high-throughput …
expressing Renilla luciferase (Rluc; rOC43-ns2Del-Rluc) facilitates high-throughput …