… of single-molecule FRET … single-molecule FRET and other advanced applications are
available elsewhere 4,5,6 . We begin with a rationale for using FRET, quickly review basic FRET …

… Single-molecule experimental techniques can solve many of … Many studies of protein machines
at the single-molecule level have … A second group of single-molecule methods, based on …

… and versatile for single-molecule sensing applications … a practical guide to their use,
focusing on the single-molecule … to perform successful single-molecule experiments, including …

… At the single-molecule level FRET is sensitive to heterogeneous subpopulations, can …
inter-laboratory benchmarking study 5 , absolute FRET efficiencies can be used to infer precise …

… Single-molecule FRET measurements were combined with modeling to allow for the
determination of rate constants for the initial formation of Type A oligomers and subsequent …

… This in turn makes single-molecule FRET an interesting tool for dynamic structural …
single-molecule FRET spectroscopy and the added information it gives compared to ensemble FRET …

… However, currently, only a couple of FRET pairs can be reliably measured on a single object…
single-molecule FRET for structural analysis of biomolecules. Here, we present an approach …

… FRET efficiency E values from the photon counts detected in the donor (D) and acceptor (A)
detection channels during a single-molecule … and exclusion of single-molecule events from …

… Often, conformational changes and molecular interactions are difficult or impossible to
synchronize, or too rare or transient to detect using ensemble FRET. Single-molecule FRET (…

… (60−62) Here, we show that ZMWs can also enable the detection of single-molecule
FRET well beyond the classical 10 nm barrier with classical fluorescent dyes constructs. This …