… repair (HDR) occurs (15). We previously achieved efficient genetic engineering for mutagenesis
and gene … In the present study, we developed efficient genetic engineering techniques …

… Indeed, it is a realistic possibility that the complete genome of a filamentous fungus will
be determined at the nucleotide level, with Aspergillus nidulans as the strongest …

… DNA constructs for different selection and/or tagging. To demonstrate utility and efficiency of
… – a subunit of the highly conserved Aspergillus nidulans CCAAT binding complex that plays …

… Our CRISPR/Cas9 procedure combines Cas9 expression driven by the constitutive
Aspergillus nidulans tef1-promoter from an autonomously replicating plasmid (pFC332; [32]) with …

… Efficient strain engineering requires genetically tractable hosts. Several methods have
been established to genetically engineer filamentous fungi such as protoplast transformation, …

… groups of fungi can be transformed, and the genetic manipulation of … In this chapter, we focus
on five filamentous fungi comprising … or agriculture, ie Aspergillus niger, Aspergillus oryzae, …

… Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic
… For example, while molecular genetic tools have been developed, including CRISPR/Cas9…

… for Aspergillus nidulans can be used for CRISPR based gene editing in filamentous fungi. We
… Even model fungi, where efficient gene targeting has been implemented via eg elimination …

… , which contains a fungal expression cassette with the Aspergillus nidulans gpdA promoter
(Punt et al. 1992), a multiple cloning site (MCS), and the A. nidulans trpC terminator [trpC(t)] (…

… Filamentous fungi occupy different niches in natural habitats and play a critical role in the …
, elucidation of fungal gene functions by high-throughput analyses requires efficient gene-…