Stabilities and instabilities in transgene expression

P Meyer - Transgenic plant research, 2022 - taylorfrancis.com
… to the presence of multiple copies of the transgene or homologous … its transcription, or by
post transcriptional processes that … High frequency, heat treatment-induced inactivation of the …

Gene regulation at transcriptional and posttranscriptional levels to combat salt stress in plants

A Singh, A Roychoudhury - Physiologia Plantarum, 2021 - Wiley Online Library
… In recent times, genetic engineering has been largely followed to confer salt tolerance
through introgressions of single transgenes or stacking multiple transgenes. However, most of …

The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana

Q Abbas, I Amin, S Mansoor, M Shafiq… - Virusdisease, 2019 - Springer
… TGS is involved in maintaining genome integrity by preventing rearrangement in centromeric
and telomeric repeats and by suppressing the activity of transposons and other invasive …

Good News for Nuclear Transgene Expression in Chlamydomonas

M Schroda - Cells, 2019 - mdpi.com
Suppression of SET3 by RNAi released the transcriptional silencing of tandemly repeated
transgenes … indeed reported an increased frequency of high-level transgene expression in a …

[PDF][PDF] The sound of silence: Transgene silencing in mammalian cell engineering

A Cabrera, HI Edelstein, F Glykofrydis, KS Love… - Cell systems, 2022 - cell.com
… formation, DNA methylation, and posttranslational modifications (… or characterization of
multiple DNA elements (promoter, … terminal repeats act as targets for transcriptional repression. …

Plant DNA Repair and Agrobacterium T−DNA Integration

SB Gelvin - International journal of molecular sciences, 2021 - mdpi.com
… individual transgenic plants each harboring a different T-DNA … that multiple DNA repair
pathways may contribute to T-DNA … 3′ end of the broken plant DNA following VirD2 removal. (C) …

[PDF][PDF] Regulation of rice tillering by RNA-directed DNA methylation at miniature inverted-repeat transposable elements

L Xu, K Yuan, M Yuan, X Meng, M Chen, J Wu, J Li… - Molecular plant, 2020 - cell.com
… In this study, we find that RdDM suppresses rice tillering. One … tillering not through
transcriptional repression or activation of … All of the transgenic plants generated in this study are in …

Comparative analysis of epigenetic inhibitors reveals different degrees of interference with transcriptional gene silencing and induction of DNA damage

A Nowicka, B Tokarz, J Zwyrtkova… - The Plant …, 2020 - Wiley Online Library
… Therefore, we repeated the experiment with a higher … except for DAC, which significantly
increased HR frequency. … degree of TGS repression and induction of DNA damage by cytidine-…

Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

T Baier, N Jacobebbinghaus, A Einhaus… - PLoS …, 2020 - journals.plos.org
… efficiency from removal of E1 and … transgene DNA into the nuclear genome, individual
transformants contain different chromosomal integration sites and potentially underwent multiple

Allele-selective transcriptional repression of mutant HTT for the treatment of Huntington's disease

B Zeitler, S Froelich, K Marlen, DA Shivak, Q Yu, D Li… - Nature medicine, 2019 - nature.com
… d, Frequency of CAG repeat lengths in the HD population. HD patient allele … Having
established selective mHTT repression in several contexts, we evaluated ZFP-B in transgenic R6/2 …