Condensin pinches a short negatively supercoiled DNA loop during each round of ATP usage

B Martínez‐García, S Dyson, J Segura, A Ayats… - The EMBO …, 2023 - embopress.org
… , extrudes DNA loops using an ATPdependent mechanism that … , such external supercoils
could imply that condensinDNA … Here, using cryo‐EM and biochemical experiments we show …

Condensin-driven loop extrusion on supercoiled DNA

E Kim, AM Gonzalez, B Pradhan… - Nature structural & …, 2022 - nature.com
… electron-imaging 13 studies of condensin I from Xenopus … in an ATP-dependent manner.
Finally, we discuss how our in … for positively supercoiled DNA with those for relaxed DNA in the …

The regulation of DNA supercoiling across evolution

A Duprey, EA Groisman - Protein Science, 2021 - Wiley Online Library
… without melting DNA, as positively supercoiled DNA is more … and condensin (for an in-depth
recent review, see Reference 64). … ATP: the DNA-compacting DNA gyrase is ATP dependent, …

Keeping intracellular DNA untangled: A new role for condensin?

J Roca, S Dyson, J Segura, A Valdés… - …, 2022 - Wiley Online Library
… double-stranded DNA segment in an ATP dependent manner [ … similar to a fractal globule,
[ 13, 14 ] such that any segment of … However, in biological systems, specific DNA sequences …

All eukaryotic SMC proteins induce a twist of-0.6 at each DNA-loop-extrusion step

R Janissen, R Barth, IF Davidson, M Taschner… - bioRxiv, 2024 - biorxiv.org
… , condensin, cohesin, and SMC5/6, which are ATP-dependent motor … Early biochemical
reconstitutions of condensin from … proteins induce positive supercoiling of genomic DNA in vivo in …

[HTML][HTML] Cell cycle-dependent control and roles of DNA topoisomerase II

JH Lee, JM Berger - Genes, 2019 - mdpi.com
… topo IIβ has roles in transcriptional regulation of gene expression [13,14,15]. Topo II (or topo
IIα) … ATP-dependent formation of positive supercoils by condensin is cell cycle regulated by …

Cohesin supercoils DNA during loop extrusion

IF Davidson, R Barth, S Horn, R Janissen, K Nagasaka… - bioRxiv, 2024 - biorxiv.org
… Chloroquine induces positive supercoiling and thus causes positively supercoiled plasmids
… , ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication

[HTML][HTML] Looping the genome with SMC complexes

E Kim, R Barth, C Dekker - Annual review of biochemistry, 2023 - annualreviews.org
ATP-dependent activity of purified condensin to be observed in in vitro biochemistry analysis
was that 13S condensin … extracts introduced positive supercoils into a circular DNA in the …

[HTML][HTML] Purified Smc5/6 complex exhibits DNA substrate recognition and compaction

P Gutierrez-Escribano, S Hormeño… - Molecular Cell, 2020 - cell.com
biochemical and biophysical activities. Purified Smc5/6 exhibits … DNA topologically
with affinity for supercoiled and catenated … plectonemes and can compact DNA in an ATP-dependent

PICH and TOP3A cooperate to induce positive DNA supercoiling

AH Bizard, JF Allemand, T Hassenkam… - Nature Structural & …, 2019 - nature.com
… We propose that this positive supercoiling provides an … to supercoiling induced by
nucleosomes and Condensin 22 , … of the DNA in an ATP-dependent manner (Fig. 4a). In a nicked …