Genetic Manipulation and Transformation Methods for Aspergillus spp.

YE Son, HS Park - Mycobiology, 2021 - Taylor & Francis
… Species of the genus Aspergillus have a variety of effects on … and transformation methods
for species of the genus Aspergillus. … manipulation and fungal transformation are described in …

[HTML][HTML] Establishment of a new and efficient Agrobacterium-mediated transformation system in the nematicidal fungus Purpureocillium lilacinum

CT Binh, HD Thai, BTV Ha, VT Tran - Microbiological Research, 2021 - Elsevier
… a new Agrobacterium tumefaciens-mediated transformation (… or for T-DNA insertion
mutagenesis in the agriculturally important … reesei, Aspergillus aculeatus, and A. oryzae with …

Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacus

R Gabriel, J Prinz, M Jecmenica… - Biotechnology for …, 2020 - Springer
… report Agrobacterium tumefaciens-mediated transformation (… similarities to the regulatory
system in Aspergillus niger [15]. … An insertion of 190 bp was found in FOAR1, which turned out …

A component of the septation initiation network complex, AaSepM, is involved in multiple cellulose-responsive signaling pathways in Aspergillus aculeatus

R Tsumura, K Sawada, E Kunitake, J Sumitani… - Applied Microbiology …, 2021 - Springer
… From a random insertional mutagenesis library generated by Agrobacterium tumefaciensmediated
transformation of A. aculeatus, the ClbR transcription factor and dipeptidyl peptidase …

[PDF][PDF] Development of an Efficient and Stable Transformation System for Aspergillus Oryzae Based on the pyrG Gene

C Zhou, Y Wan, H Yao, H Chen, Y Xiao, Z Tang… - 2020 - pdfs.semanticscholar.org
… Strain P1 contain 1 bp deletion and 1 bp insertion at the target sequence of the pyrG gene(Fig. …
protoplast transformation ATMT Agrobacterium tumefaciens-mediated transformation FDA …

Functional Genomics of Aspergillus oryzae: Strategies and Progress

B He, Y Tu, C Jiang, Z Zhang, Y Li, B Zeng - Microorganisms, 2019 - mdpi.com
… The CRISPR/Cas9 system has been successfully adapted to filamentous fungi such as
Aspergillus aculeatus, Aspergillus fumigatus, and A. oryzae [52,53]. The procedure of this system …

Construction of Aspergillus Oryzae food-grade expression system based on auxotrophic markers

Y Qin, C Zhou, W Jin, H Yao, H Chen, Y Wan… - Food …, 2021 - Taylor & Francis
Aspergillus oryzae is an ideal host for expressing heterologous and homologous genes. Due
to the risk of antibiotics to food safety, it is banned for use in resistance screening with food-…

The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool

GM Daba, FA Mostafa, WA Elkhateeb - Bioresources and Bioprocessing, 2021 - Springer
… The CRISPR/Cas9 system has been successfully adapted to filamentous fungi such as
Aspergillus aculeatus, Aspergillus fumigatus, and A. oryzae (Nødvig et al. 2015; El-Sayed et al. …

Establishment, optimization, and application of genetic technology in Aspergillus spp.

J Gao, H Liu, Z Zhang, Z Liang - Frontiers in Microbiology, 2023 - frontiersin.org
Aspergillus is widely distributed in nature and occupies a … With the deepening of genomics
exploration, more Aspergillus … realize the ideal functional transformation. Available genetic …

Aspergillus spp., a versatile cell factory for enzymes and metabolites: Interventions through genome editing

A Madhavan, A KB, R Sindhu, P Binod, MK Awasthi… - 2022 - nopr.niscpr.res.in
… mediated transformation10 were successfully done in Aspergillus. The introduced DNA …
insertion of transforming DNA and generation of new deletions associated with transformation