[HTML][HTML] An RNA in situ hybridization protocol optimized for monocot tissue
NR Zöllner, M Bezrutczyk, R Laureyns, H Nelissen… - STAR protocols, 2021 - Elsevier
STAR protocols, 2021•Elsevier
RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we
provide an optimized protocol for maize leaf tissue, though it can be applied to other plant
tissues such as shoot apical meristems, embryos, and floral organs. We generate three> 100
bp unique antisense probes for each gene of interest and hybridize them to tissue sections.
For complete details on the use and execution of this protocol, please refer to Bezrutczyk et
al.(2021).
provide an optimized protocol for maize leaf tissue, though it can be applied to other plant
tissues such as shoot apical meristems, embryos, and floral organs. We generate three> 100
bp unique antisense probes for each gene of interest and hybridize them to tissue sections.
For complete details on the use and execution of this protocol, please refer to Bezrutczyk et
al.(2021).
Summary
RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense probes for each gene of interest and hybridize them to tissue sections.
For complete details on the use and execution of this protocol, please refer to Bezrutczyk et al. (2021).
Elsevier
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