Myocardial infarction is one of the major causes of global premature mortality and morbidity. As a traditional medicine, Curcuma longa have been used to reduce risk of cardiovascular disease. This study was designed to evaluate the cardioprotective effect of Curcuma longa in isoproterenol induced myocardial infarction in rats. Total 28 Wistar albino male rats were divided into BC (base line control group), ISP (isoproterenol treated control group), CLP-ISPT (Curcuma longa pretreated and isoproterenol treated group) and AP-ISPT (amlodipine pretreated and isoproterenol treated group). At the end of experiment, all the rats were sacrificed. Blood samples were collected from the heart. Serum Aspartate Aminotransferase (AST), Lactate Dehydrogenase (LDH) and Creatine Kinase-MB (CK-MB) levels were estimated to assess myocardial injury. The statistical analysis was done by one-way ANOVA, followed by Bonferroni test. In this study, mean serum AST, LDH and CK-MB levels were significantly (P