… enabling the release of the nucleic acid. Moreover, protonation of … and the escape of the
nucleic acids from endosomes. These … mitigated the solubility issues and enhanced transfection. …

… issues … nucleic acids will not be able to migrate freely through agarose during gel
electrophoresis, in contrast to the unencapsulated NAs. Consequently, encapsulated nucleic acids …

… lipid-nucleic acid complexes have been developed in order to address some of these issues
… the cellular pathway and transfection mechanisms of different lipid-nucleic acid complexes. …

… direct the nucleic acid at a specific site. Therefore, the successful delivery of RNA-based
nucleic acid is still a challenge that blocks the breakthroughs in gene therapy. Improving the …

… , the challenges to its application, namely the nucleic acids’ … of lipid to DNA required to
achieve maximum transfection in … Other methods consist in hydrating a lipid film which already …

… They found that the transfection and load of siRNA remained almost the same after 1 h of …
[56] utilized ginger lipids to fabricate liposomes via a thin-film hydration method to transfer …

… However, the targeted delivery and endosomal escape of RNA-LNPs remain challenging
for RNA delivery systems, which highlights the need for the development of safe, effective and …

… [29], who have created aerosolizable dry powder of lipid nanoparticles by thin-film freeze-drying
(… of difficulties encountered in nucleic acid packaging and poor transfection efficiency. To …

… and intracellular challenges such as nucleic acid breakdown … in the use of cationic lipids for
transfection [Citation4]. In 1989 ( … , lipids dissolved in organic solvents are dried into thin films …

… “nucleic acids” here includes various types of nucleic acid … the successful modification of
lipids for transfection. The historic … Challenges and advances of active targeted nanoparticles …