… many problems associated with delivering nucleic acids to the … on intracellular delivery/transfection
of nucleic acids in the … The dry lipid film can be hydrated by dispersing PNP or by …

… However, a universally applicable transfection strategy that … Efficient transfer into primary
tissue is even more challenging… Subsequently, dried lipid films were dispersed in 20 mM 2-(4-…

… While cationic lipids in LNPs enhance the transfection efficiency, … lipids and LNP formulation
which can address issues of … Dry lipid films were hydrated with DFW at 65 C for 1 h. MLVs …

… conditions, transfecting hair follicles using the commercially available transfection system …
for mitochondrial delivery face a number of problems, including cell internalization, size …

… To our knowledge, this is the first time the transfection … lipid formulation was prepared
by the thin-film hydration method by producing a lipid film with the cationic lipid (OH4), the co-lipid …

… challenging due to their complex structure and high molecular weight (between 300 and
1,000,000 Da) in addition to stability issues … improved uptake and transfection of A431 cells in …

… escape and transfection efficiency of LNPs by modifying lipid … phase, nucleic acids are
sandwiched between lipid bilayers … The dried lipid film was hydrated with sterile Milli-Q water and …

… challenges still remain, most notably in effective delivery of the therapeutic nucleic acid (NA) …
The pDNA-XMaN6 LNPs had the highest transfection efficiency, transfecting 91% of …

… transfection using this system is efficient and safe, requiring amounts of nucleic acid with an
… [ 39 ] Alternatively, LbL films containing intracellular nucleic acid cargoes have been shown …

… need to deal with the issues of reproducibility, scalability of … lipids for nucleic acid complexation,
helper lipids and a PEG-… , the gold standard for the transfection of lipid-based systems. …