… transfection using this system is efficient and safe, requiring amounts of nucleic acid with an
… [ 39 ] Alternatively, LbL films containing intracellular nucleic acid cargoes have been shown …

… need to deal with the issues of reproducibility, scalability of … lipids for nucleic acid complexation,
helper lipids and a PEG-… , the gold standard for the transfection of lipid-based systems. …

… well-known from nanoparticle-mediated transfection. Beyond direct membrane fusion with
… These issues can be addressed by their formulation in nanoparticles. Notably, a larger body …

… delivery system of exogenous nucleic acid remains an ongoing challenge. In recent …
transfection, the risk of confounding the outcomes by analyzing the presence of foreign nucleic acid …

… (core production and subsequent lipid film hydration) is complicated … issues should restrict
the clinical use of non-viral vectors. The dilemma of toxicity and effectiveness of transfection is …

… Because, as discussed above, nucleic acid release is crucial to successful transfection as …
hours and improved transfection of glioblastoma cells compared to LbL films based on PBAE …

… We outline challenges and opportunities for advanced … delivery of drugs including nucleic
acid derived therapeutics. … using commercially available lipid-based transfection reagents as …

… several difficulties such as the toxicity of viral vectors used in delivery of nucleic acids and the
… internalized into tumor cells and show limited transfection efficiency. Efforts are ongoing to …

… challenges and setbacks are still limiting their clinical implementation. In spite of high
transfection efficiency, viral transfection … , and cationic lipids interacting with nucleic acids. Their …

… unmet challenges in successful delivery of nucleic acids and … Currently, thin film hydration
technique is not recommended … in the transfection efficiency of the nucleic acid payload …