… by introducing exogenous nucleic acids such as messenger RNA (mRNA), small interfering
… problems existing in RNA delivery in vivo, which is the key for improving the efficacy of RNA …

… lipid concentration and nucleic acids affects transfection … evaporation for the generation of
a thin lipid film. A total of 1 mL … For instance, the instability of vaccines often poses challenges …

… through lipid-based transfection remains a key challenge for the development of RNA
therapeutics. In … The molecular and cellular processes of nucleic acid nanoparticles share …

… in the development of nucleic acid therapeutics, in large part … (12) First, we use lipid
molecules to produce a thin film. Giant … to balance the transfection efficiency of lipid-nanoparticles …

… These issues limit their practical utility in vivo.3-5 Thus, the … and transfection activities of two
samples of the same lipid (N’-… can penetrate into the liquid lipid film, but will be squeezed-out …

… nucleic acids to target cells remains a significant challenge … contribute to their low transfection
efficiency and cytotoxicity, … ) or pressure (eg dry lipid film hydration followed by extrusion or …

… lipid formulations are reported to be excellent transfection … The liposomes obtained by lipid
film hydration methods are generally … These problems can be overcome by encapsulating the …

… mitochondrial transfection system for delivering nucleic acids … of the difficulties associated
with delivering nucleic acids to … , followed by packaging via the lipid film hydration method, as …

… cells transfected by free locked nucleic acids [56], and 30% of transfected cells with solid/lipid …
Instead of mixing polyplexes with a dried lipid film followed by sonication and extrusion, we …

… lipids, cholesterol and its nucleic acid payload, in contrast, the LNP surface (ie lipid-water
interface) is rich in helper phospholipids and lipid-… until the entire lipid film was fully suspended …