Generation of cationic nanoliposomes for the efficient delivery of in vitro transcribed messenger RNA

T Michel, A Link, MK Abraham, C Schlensak… - JoVE (Journal of …, 2019 - jove.com
… their complexation and transfection efficacies using synthetic … transfection rate with negatively
charged nucleic acids 38 . … system, two of the key problems of drug delivery can be solved…

Auto-associative lipid-based systems for non-viral nucleic acid delivery

V Escriou, N Mignet, A Miller - Introduction to Systems Biology; …, 2019 - books.google.com
… expression ( transfection ) of therapeutic nucleic acids such as … of cationic liposomes: A thin
lipid film on the walls of a glass … a chemical set of solutions looking for biological problems. …

Overcoming the delivery barrier of oligonucleotide drugs and enhancing nucleoside drug efficiency: The use of nucleolipids

X Zhou, S Wang, Y Zhu, Y Pan… - Medicinal Research …, 2020 - Wiley Online Library
… the transfection abilities offered by the nucleic acids of nucleolipids are ignored; only a few
works discuss it. The formation of nucleolipid/nucleic acid … and transportation problems that …

[HTML][HTML] Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems

H Elsana, TOB Olusanya, J Carr-Wilkinson, S Darby… - Scientific reports, 2019 - nature.com
… They can overcome major viral issues such as … Liposomes can entrap different molecules
such as nucleic acids and … The aim of this research is to improve cationic liposome transfection

Impact of net charge, targeting ligand amount and mRNA modification on the uptake, intracellular routing and the transfection efficiency of mRNA lipopolyplexes in …

C Delehedde, I Ciganek, N Rameix, N Laroui… - International Journal of …, 2023 - Elsevier
… cell specificity is one of the challenges that must be tackled to … LPR exhibits higher colloidal
stability and nucleic acid … Then, the lipid film was further sonicated at 37 kHz by using an …

[引用][C] Microfluidics for size controlled cationic liposome DNA complexes: going beyond the universal transfection curve

CJO Ferreira, BFB Da Silva, MECD Real, ODC Botelho

[HTML][HTML] Translating a Thin-Film Rehydration Method to Microfluidics for the Preparation of a SARS-CoV-2 DNA Vaccine: When Manufacturing Method Matters

A Peletta, E Prompetchara, K Tharakhet, P Kaewpang… - Pharmaceutics, 2022 - mdpi.com
… ], with one of the main challenges being vaccine accessibility and … of fragile molecules such
as nucleic acids [13]. Previously, a … to be of lower transfection capacity. No fluorescence was …

Layer-by-Layer technique as a versatile tool for gene delivery applications

DS Linnik, YV Tarakanchikova, MV Zyuzin… - Expert Opinion on …, 2021 - Taylor & Francis
nucleic acid release and cell transfection via electroporation from LbL-assembled film modified
with nucleic acid … at the same time, as the main challenges for the development of ex vivo …

Mechanisms and challenges of nanocarriers as non-viral vectors of therapeutic genes for enhanced pulmonary delivery

H Wang, L Qin, X Zhang, J Guan, S Mao - Journal of Controlled Release, 2022 - Elsevier
… Still, issues of low transfection efficiency and poor tissue targeting of non-viral vectors need
… charged nucleic acids via electrostatic interactions. However, the transfection efficiency of …

[HTML][HTML] Fabrication of active targeting lipid nanoparticles: Challenges and perspectives

I Menon, M Zaroudi, Y Zhang, E Aisenbrey… - Materials Today Advances, 2022 - Elsevier
… Some groups tried to use antibodies aqueous solutions to hydrate plain lipid films [38]. …
another popular technique for lipid nanoparticle formulation, especially nucleic acid-loaded …