Use of a multiplex PCR assay to assess the presence of Treponema pallidum in mucocutaneous ulcerations in patients with suspected syphilis

PA Grange, A Jary, C Isnard, S Burrel… - Journal of clinical …, 2021 - Am Soc Microbiol
… Allplex genital ulcer real-time PCR multiplex assay for detecting Treponemareal-time
multiplex PCR assay for simultaneous detection of the genomes of Treponema pallidum, herpes …

[HTML][HTML] A novel nested real-time polymerase chain reaction for Treponema pallidum DNA in syphilis biospecimens

Y Zhang, X Dai, Z Ren, H Lin, W Cao… - Sexually transmitted …, 2019 - journals.lww.com
… All procedures used in this study were approved by the medical ethics committee of the …
for real-time PCR, and we did not analyze syphilis diagnoses for positive DNA tests using NR-…

[HTML][HTML] … retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with …

E Vrbová, L Mikalová, L Grillová, P Pospíšilová… - PLoS …, 2020 - journals.plos.org
… of PCR detection of syphilis treponemes in clinical samples, this study shows that nested PCR
can improve syphilis diagnostics, … Clinical value of Treponema pallidum real-time PCR for …

Quantified detection of Treponema pallidum DNA by PCR assays in urine and plasma of syphilis patients

C Wang, X Zheng, Z Guan, D Zou, X Gu… - Microbiology …, 2022 - Am Soc Microbiol
… Though the diagnosis of syphilis is mainly based on clinical manifestations combined with
… investigate the detection rate and loads of T. pallidum DNA in urine in syphilis patients at …

Prevalence and Predictors of Oral Treponema pallidum Detection by Quantitative Polymerase Chain Reaction in Early Syphilis

JA Dionne, L Giacani, A Tamhane… - The Journal of …, 2024 - academic.oup.com
… acid amplification testing (NAAT) for Chlamydia trachomatis and … in Treponema pallidum
polymerase chain reaction (PCR) … to determine clinical and host factors associated with oral T. …

A New Specimen for Syphilis Diagnosis: Evidence by High Loads of Treponema pallidum DNA in Saliva

C Wang, Z Hu, X Zheng, M Ye, C Liao… - Clinical Infectious …, 2021 - academic.oup.com
Treponema pallidum DNA detection rates in saliva and plasma were 31.0% (9/29) and
51.7% (15/29) in primary syphilis (P = .11), 87.5% (63/72) and 61.1% (44/72) in secondary …

[HTML][HTML] Laboratory diagnostic tools for syphilis: current status and future prospects

Y Luo, Y Xie, Y Xiao - Frontiers in cellular and infection microbiology, 2021 - frontiersin.org
… Therefore, a sensitive method for detecting T. pallidum is fundamental for the prompt
diagnosis of syphilis. Morphological … Clinical Value of Treponema pallidum Real-Time PCR for …

PCR detection for syphilis diagnosis: Status and prospects

C Zhou, X Zhang, W Zhang, J Duan… - Journal of clinical …, 2019 - Wiley Online Library
Syphilis, a re-emerging public health problem worldwide caused by Treponema pallidum
subsp pallidum (T… In this review, five studies about real-time PCR for syphilis diagnosis are …

Detection of Treponema pallidum DNA During Early Syphilis Stages in Peripheral Blood, Oropharynx, Ano-Rectum and Urine as a Proxy for Transmissibility

SA Nieuwenburg, HCA Zondag… - Clinical Infectious …, 2022 - academic.oup.com
… a polymerase chain reaction (PCR) targeting the polA gene of Treponema pallidum (TP-PCR
Moreover, an ulcer swab for DNA extraction and real-time PCR targeting the polA gene was …

Refining timely diagnosis of early syphilis by using Treponema pallidum PCR or IgM immunoblotting next to conventional serology for syphilis

J Flipse, AM Niekamp, A Dirks… - Journal of Clinical …, 2023 - Am Soc Microbiol
… In this study, added value was defined as finding more cases of syphilis or preventing
overtreatment (… Clinical value of Treponema pallidum real-time PCR for diagnosis of syphilis. J …