… , using ImageJ, use the Rectangular Selections tool to select the first lane of the gel to quantify.
Go to Analyze| … , we were able to validate the semi-quantitative nature of the IF approach. …

… mechanisms remains primarily semi-quantitative. 56 … that can influence Western blot
analysis and 79 interpretation… like ImageJ can aid in the detection of signal 122 saturation. …

… ) proteins is a commonly used, semi-quantitative method to … RBC using the Oval selection
tool within the ImageJ software. … obtained primary antibodies (eg, western blot). First, a primary …

… Here we present a modified protocol of Horita et al. [3]. The … The method described here is
simple, semi-quantitative and … quantitated by densitometry using imageJ software and levels …

… Band intensities were quantified with ImageJ software. ***p < 0.001; **p < 0.01; … western
blot technique is only able to produce semi-quantitative data, which means that the technique …

… is a semi-quantitative technique, MMP-3 at various protein … ImageJ software showed the
upregulation of Annexin A2 in C6-13 … validated by western blot analysis (Figure 3.4). In this case, …

… , mRNA and protein isoform immunoblotting measurements (… Cells were manually counted
in ImageJ using overlay … expression using semi-quantitative real-time PCR analysis (semi-RT-…

… human PDZ-proteins were undetected by the MS approach, we used western blot to further
… splicing, post-translational modifications, or binding to third-party proteins or other ligands. …

… protein was roughly proportional to its abundance. A popular iteration of this method known
as Exponentially Modified Protein … lane were obtained using ImageJ gel analysis tools. The …

… with Fiji ImageJ, using automated thresholding and the “analyze … In summary, the results from
the immunoblots reveal that both … , work and computation. Thus, we explored an alternative …