… species can be preserved with this encapsulation-dehydration technique and pretreatment
prior to … Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium sims.(…

… storage of immature seeds in liquid nitrogen was studied. Immature seeds can germinate in
… up to the stage of embryo swelling or protocorm without rhizoids. The maximum staining of …

… of protocorms and protocorm-like bodies mostly employ desiccation, PVS2 vitrification or
encapsulation-dehydration. … for both protocorms and PLBs of orchids, however, a few reports on …

… tolerant materials, such as seeds and protocorm-like bodies (PLBs)… understanding of
the cryobiology of orchids. Some previous … graminifolia protocorm using encapsulation-dehydration …

… All types of in vitro-cultured materials that have been used for … regeneration through
cryopreserved seeds and protocorms, this … In this work, the encapsulation-dehydration technique …

… have focused on asymbiotic propagation of orchids from seeds [3, 4]… orchids to be
cryopreserved by encapsulation-dehydration. … of Vanda coerulea protocorms after …

… explants (ie somatic embryos, protocorm-like bodies, shoot tips, bulblets and axillary buds). …
Although the encapsulation-dehydration cryopreservation has been successfully applied to …

… It is widely used, for example, with orchids (seeds and PLBs) [64,65]. Encapsulation-dehydration
has … Conversely, PVS2-induced vitrification was lethal for the protocorms of Paralophia …

… coat broke; stage 3: protocorm showed vegetative apex; stage 4: development of the first
leaf; … In this study, the MC was nearly 19.2% MC of Oncidium bifolium seed cryopreservation (…

… encapsulation-dehydration approach uses the in vitro cultured orchid plant tissues, seeds, …
-RNAi construct into the protocorm-like bodies (PLBs) of Oncidium hybrid, resulting in down-…