… Ectopic integration could also be mutagenic due to unintended gene disruption, and the risk
… neoformans possesses several classic virulence traits that enable it to survive in the host, …

… , we describe an STM-based murine infectivity assay method using a collection of mutants
harboring a unique DNA tag to identify genes involved in infectivity and/or virulence of C. …

… insertion mutation was present in clade A (Table S2). Although the majority of these variants
are in genes … Using gene complementation, we clearly show the virulence defect in the itr4Δ …

… human pathogen Cryptococcus causes genomic mutations in a … is a key virulence determinant
for pathogenic fungi during the … Cryptococcus neoformans (serotype A) is the predominant …

… We observed attenuation in virulence only in an inhalation model where C. neoformans is
… The plasmid was sequenced to confirm no mutations were introduced during the cloning …

… for random insertional mutagenesis or targeted gene … than the human pathogen Cryptococcus
neoformans, and are very … vir genes required for virulence (ie, transfer of DNA into the …

… , the relationship among genes, regulatory mechanisms, virulence, and treatment is … to
better understand how C. gattii causes disease and to characterize hypervirulent C. gattii …

… It also helps to understand the virulence and drug resistance … utilizing the technique for an
insertion mutagenesis study [34]… The recently discovered clustered regularly interspaced short …

… neoformans clinical isolates with increased mutation rates … virulence and drug resistance,
similar to the effects of the Ac/Ds elements of maize, the first transposable elements discovered …

… Facile genetic manipulation is a cornerstone of the study of microbial pathogens, allowing
the use of reverse genetics to dissect gene function, discover novel virulence determinants, …