Oxygen as a master regulator of human pluripotent stem cell function and metabolism

K Nit, M Tyszka-Czochara… - Journal of Personalized …, 2021 - mdpi.com
… generated equivalents of human embryonic stem cells (hESCs… due to their origin and the
necessity to destroy an embryo. … be explained by the higher oxygen concentration (5% O 2 ) …

HYPOXIA AND REPRODUCTIVE HEALTH: Hypoxic regulation of preimplantation embryos: lessons from human embryonic stem cells

FD Houghton - Reproduction, 2021 - rep.bioscientifica.com
… It became apparent that not only the concentration of oxygen used, but also the stage at …
In addition, the derivation and culture of hESCs under physiological oxygen (5% oxygen) has …

Modeling X chromosome inactivation using t5iLA naive human pluripotent stem cells

Y Shang, N Wang, H Wang, C An, W Sun - BMC biology, 2024 - Springer
… , we utilized four primed pluripotent human embryonic stem cell lines, WIBR3 MGT and …
in hybridization buffer using 100 μL to achieve a final concentration of 20 ng/μL. Finally, the …

Preventing erosion of X-chromosome inactivation in human embryonic stem cells

M Cloutier, S Kumar, E Buttigieg, L Keller, B Lee… - Nature …, 2022 - nature.com
inactivation upon prolonged culture. Here, we investigate the sources of X-inactivation instability
by deriving … with LiCl media on HFFs under physiological oxygen concentration (5% O 2 …

Overcoming autocrine FGF signaling-induced heterogeneity in naive human ESCs enables modeling of random X chromosome inactivation

C An, G Feng, J Zhang, S Cao, Y Wang, N Wang, F Lu… - Cell stem cell, 2020 - cell.com
… Together, these findings enable derivation of homogeneous naive hESCs and establish a
Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen

Chemically defined media can maintain pig pluripotency network in vitro

KH Choi, DK Lee, SW Kim, SH Woo, DY Kim, CK Lee - Stem cell reports, 2019 - cell.com
… Our findings will facilitate both the development of large animal models for human stem cell
Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen

Hypoxia as a driving force of pluripotent stem cell reprogramming and differentiation to endothelial cells

P Podkalicka, J Stępniewski, O Mucha… - Biomolecules, 2020 - mdpi.com
… may facilitate the derivation of embryonic stem cells (ESCs) … degradation under normal
oxygen concentration through … It is usually composed of mitotically inactivated mouse embryonic

Nicotinamide adenine dinucleotide induces a bivalent metabolism and maintains pluripotency in human embryonic stem cells

JG Lees, DK Gardner, AJ Harvey - Stem Cells, 2020 - academic.oup.com
… are therefore critical to derive ATP oxidatively through the … concentration range (Supporting
Information Figure S1B-D). … H3K27me3 foci characteristic of an inactivated X-chromosome in …

Epigenetic aberrations in human pluripotent stem cells

S Bar, N Benvenisty - The EMBO journal, 2019 - embopress.org
… hPSCs can be classified by the method used to derive them. Human embryonic stem cells
(h… Yet, this requires hESCs to be derived under low oxygen concentration, or at least to be …

Embryo culture at a reduced oxygen concentration of 5%: a mini review

R Sciorio, GD Smith - Zygote, 2019 - cambridge.org
… This review analyzes how oxygen usage affects the physiology of the preimplantation …
Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen