Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus
L Ma, F Zeng, F Cong, B Huang, R Huang, J Ma… - Journal of virological …, 2019 - Elsevier
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was
associated with severe diarrhea disease in pigs. SADS-CoV was first detected and identified
as the causative agent of a devastating swine disease outbreak in southern China in 2017.
Routine monitoring and early detection of the source of infection is therefore integral to the
prevention and control of SADS-CoV infection. In this study, a SYBR green-based real-time
reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique was …
associated with severe diarrhea disease in pigs. SADS-CoV was first detected and identified
as the causative agent of a devastating swine disease outbreak in southern China in 2017.
Routine monitoring and early detection of the source of infection is therefore integral to the
prevention and control of SADS-CoV infection. In this study, a SYBR green-based real-time
reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique was …
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