The development of immunological assays to evaluate the level and function of antibodies induced by Klebsiella pneumoniae O-antigen vaccines

HR Wagstaffe, M Johnson, G Osman, P Martin… - Msphere, 2023 - Am Soc Microbiol
… a Luminex-based multiplex antibody binding assay and both … The lower limit of quantification
(LLOQ) was determined as 4 … from the validated Streptococcus pneumoniae OPA which …

[HTML][HTML] The development of a multiplex serological assay for avian influenza based on Luminex technology

E Germeraad, R Achterberg, S Venema, J Post… - Methods, 2019 - Elsevier
… sets is detected by fluorescent secondary antibodies, and the … We performed a phylogenetic
analysis for all HA and NA … medium using Strep-Tactin affinity chromatography (Strep-Tactin/…

[HTML][HTML] Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii

R Jeske, L Dangel, L Sauerbrey, D Frangoulidis… - Microorganisms, 2021 - mdpi.com
… the quantitative high-throughput detection of antibodies to … coupled to a set of
fluorescence-labelled glutathione casein-… burnetii multiplex serology assay, antibody responses to …

[HTML][HTML] Development of a diagnostic assay by three-tube multiplex real-time PCR for simultaneous detection of nine microorganisms causing acute respiratory …

XW Jiang, TS Huang, L Xie, SZ Chen, SD Wang… - Scientific Reports, 2022 - nature.com
multiplex PCR methods required post-PCR analysis, leading … required for the multiplex
fluorescent nested PCR method … and Streptococcus pneumoniae if identified by this multiplex PCR…

Acute Rheumatic Fever is Associated with a Network of Inflammatory Markers and Exaggerated IgG3 Responses to the Group a Streptococcus M-Protein

N Lorenz, R McGregor, AL Whitcombe… - Available at SSRN … - papers.ssrn.com
… To that end, this study encompasses extensive analysis of … GAS antibody response, a
combination of multiplex bead-… exaggerated GAS-specific antibody responses, elevated total IgG3 …

Qualification and Clinical Validation of an Immunodiagnostic Assay for Detecting 11 Additional Streptococcus pneumoniae Serotype–specific Polysaccharides in …

WV Kalina, V Souza, K Wu, P Giardina… - Clinical Infectious …, 2020 - academic.oup.com
multiplex urinary antigen detection (UAD) assay that identifies serotypes covered by the
13-valent PCV (PCV13; Prevnar 13®, Pfizer) was developed … from the determination of sensitivity …

[HTML][HTML] Investigation of the pathogen-specific antibody response in periprosthetic joint infection

V Janz, A Rakow, L Schröder, A Hofer, S Wiebe… - Infection, 2024 - Springer
… To test these hypotheses, we developed a quantitative high-… antibody levels ranging from a
factor of 7 for Streptococcus oralis (S. … Our pilot study shows that the multiplex IA is suitable for …

Streptococcus pyogenes pharyngitis elicits diverse antibody responses to key vaccine antigens influenced by the imprint of past infections.

J Osowicki, HR Frost, KI Azzopardi, AL Whitcombe… - bioRxiv, 2024 - biorxiv.org
… by a multiplex bead-based assay for IgG antibodies in sera. B… analysis between MDS
dimension 1 and IgG responses, only … participants who subsequently developed pharyngitis (red) …

[HTML][HTML] Development of A Standardized Opsonophagocytosis Killing Assay for Group B Streptococcus and Assessment in an Interlaboratory Study

S Leung, CF Collett, L Allen, S Lim, P Maniatis… - Vaccines, 2023 - mdpi.com
… in the development of a multiplexed (Luminex) immunoassayquantification of OPKA activity
in samples with low antibody … pHrodo, a fluorescent dye that increases in fluorescence in the …

Re-validation and update of an extended-specificity multiplex assay for detection of Streptococcus pneumoniae capsular serotype/serogroup-specific antigen and cell …

SD Eletu, CL Sheppard, S Rose… - Access …, 2020 - microbiologyresearch.org
developed a highly sensitive extended-specificity multiplex … (WGS) data become available
and analysis of the capsular … due to other mitis group streptococci or pneumococcal carriage …