… purity and EV yield, combined EV isolation methods clearly … are required to optimize the
various parameters (eg cross-flow … isolation of EV subpopulations and prevents EV damage (eg …

… distinguish lipoproteins from small EVs by TEM in this setting. … The different methods enrich
for distinct EV subpopulations … , different methods for EV isolation yield different products …

… These drawbacks limit dUC to become a routine clinical setting, but it is still widely used in
… preferred method for EV isolation (low yield/high purity), directing the particles to separate by …

… that guarantee scale-up of sample volume, purity, speed, yield, and tumor … and subpopulation
isolation methods that isolate and enrich subpopulation by targeting antibodies against EV …

… , yield and size distribution of EVs has revealed the highest … EV enrichment strategies to
find a method that is suitable for … , as EV subpopulation with different cargo and EV proteins …

… enrich EVs based on the reasoning presented below. This … different variants of the protocol
for their respective EV yields (… This question is important because many clinical samples are …

… This method yields single EV banding at their characteristic … has been shown to produce
preparations that are enriched … to the point of extracting subpopulations of EVs based on the …

… for sEV protein ID, purity, and overall particle yield. Our data … The remaining gas and source
parameters were adjusted as … explore EV subpopulations, as non-specific methods isolate a …

… In conclusion, the sUC EV-enrichment method is compatible with … samples, typical for clinical
settings. Still, SEC is a valuable … , the methods seem to detect a different subpopulation of …

… EV subpopulations remains a challenge. Alternative, similar, or identical experimental
approaches may often lead to substantially different EV profiling results in different laboratories. …