Standardization of bcr-abl1 p210 monitoring: From nested to digital pcr

A Jovanovski, J Petiti, E Giugliano, EM Gottardi… - Cancers, 2020 - mdpi.com
… who approach the chronic myeloid leukemia field. … the potential use of dPCR as highly
sensitive approach in CML … QXDx BCR-ABL %IS ddPCR assay. They then suggested that this …

[HTML][HTML] … Polymerase Chain Reaction Method Yields Similar Level of Sensitivity to Digital Droplet Polymerase Chain Reaction for Detection of BCR-ABL p210 …

H Asghari, C Talati, A Achille, JJ Powers, E Sahakian… - Blood, 2019 - Elsevier
… and ddPCR was performed using the QX-200 Droplet Digital PCR System and QXDx BCR-ABL
… Analysis for RNA degradation of stored samples was performed via High Sensitivity RNA …

Droplet Digital PCR for BCR–ABL1 Monitoring in Diagnostic Routine: Ready to Start?

MT Bochicchio, J Petiti, P Berchialla, B Izzo… - Cancers, 2021 - mdpi.com
… approach in chronic myeloid leukemia patients with a stable … analyzed using both ddPCR
QXDx TM BCR-ABL %IS Kit and … [3], a highly sensitive and accurate technique for BCR–ABL1 …

Comparison of droplet digital PCR versus qPCR measurements on the international scale for the molecular monitoring of chronic myeloid leukemia patients

AA Cortés, S Olmedillas, J Serrano-López… - Molecular Diagnosis & …, 2020 - Springer
… a greater sensitivity than qPCR, probably due to the high … , which encodes a chimeric
BCR-ABL tyrosine kinase that leads … -ABL1/ABL1 p210 results in IS from ddPCR and qPCR under …

[HTML][HTML] A comparison of droplet digital PCR and RT-qPCR for BCR-ABL1 monitoring in chronic myeloid leukemia

C Fava, P Berchialla, J Petiti, MT Bochicchio, B Izzo… - Blood, 2019 - Elsevier
… experiments were performed using Kit QXDxTM BCR-ABL%IS (Bio-… For RT-qPCR experiments,
BCR-ABL1 p210 was quantified … Sensitivity analysis showed that the high coefficient of …

Committed change of real-time quantitative PCR to droplet digital PCR for monitoring BCR::ABL1 transcripts in tyrosine kinase inhibitor treated CML

A Kongruang, N Limsuwanachot, S Magmuang… - …, 2023 - Taylor & Francis
… , representing high sensitivity to quantify the low abundance … Chronic myeloid leukemia
(CML) is a myeloproliferative … of RNA samples using a QXDx TM BCR-ABL %IS Kit (Bio-Rad…

… of the first Food and Drug Administration (FDA)-cleared digital droplet PCR (ddPCR) assay for BCR::ABL1 monitoring in chronic myelogenous leukemia

DN Shelton, P Bhagavatula, N Sepulveda, L Beppu… - PLoS …, 2022 - journals.plos.org
… of a droplet digital PCR based assay (QXDx BCR-ABL %IS Kit; … ™ BCR-ABL %IS Kit is an
accurate, precise, and sensitive … as t (9;22) positive CML p210. Additional patient samples were …

[HTML][HTML] Advantages of digital PCR in the detection of low abundance BCR‑ABL1 gene in patients with chronic myeloid leukemia

Z Yan, Q Sun, H Zhang, Y Han, J Qiao… - Oncology …, 2019 - spandidos-publications.com
DigitalPCR (dPCR) can be applied to detect the BCR‑ABL1 gene with high sensitivity. In …
However, RT-qPCR can lead to errors in BCR-ABL detection. As such, appropriate correction …

[HTML][HTML] Quantitative detection of T315I mutations of BCR:: ABL1 using digital droplet polymerase chain reaction

H Mu, J Zou, H Zhang - Hematology, Transfusion and Cell Therapy, 2024 - Elsevier
… lymphoblastic leukemia (ALL) and 85 patients with chronic myeloid … companies have produced
different digital PCR platforms, such … to processing using the ddPCR BCR::ABL T315I kit (…

One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia

RJ Martinez, Q Kang, D Nennig… - … of Pathology & …, 2022 - meridian.allenpress.com
… that allows for highly sensitive absolute … Droplet digital polymerase chain reaction has been
shown to be a reliable tool for monitoring clinical disease in chronic myelogenous leukemia