[HTML][HTML] Induction of integration-free human-induced pluripotent stem cells under serum-and feeder-free conditions

A Hamada, E Akagi, S Yamasaki, H Nakatao… - In Vitro Cellular & …, 2020 - Springer
… To further simplify the generation of hiPSCs and to increase the donor population, we …
generation of hiPSCs using peripheral blood mononuclear cells (PBMCs) from seven healthy adult

[HTML][HTML] Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions

S Perriot, M Canales, A Mathias, R Du Pasquier - STAR protocols, 2022 - Elsevier
… Moreover, working with erythroblasts ensures that no recombination of the TCR/BCR
genes occurs, as opposed to T cells and whole peripheral blood mononuclear cells-based …

[HTML][HTML] Efficient generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells

Y Gao, Y Cheng, J Wen, D Xiao, J Xu, C Cai, J Hu - Stem Cell Research, 2023 - Elsevier
… showed low efficiency in generating integration-free iPSCs. Relatively efficient approaches
that … Cell morphology of four reprogramming induced pluripotent stem cells. B. RT-PCR data …

[HTML][HTML] Induction of Noonan syndrome-specific human-induced pluripotent stem cells under serum-, feeder-, and integration-free conditions

A Hamada, E Akagi, F Obayashi, S Yamasaki… - In Vitro Cellular & …, 2020 - Springer
… Notably, these cells showed potential for application to genetic disorder … , using similar
procedures, we reported the induction of hiPSCs derived from peripheral blood mononuclear cells

[HTML][HTML] Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

K Vlahos, K Sourris, R Mayberry, P McDonald… - Stem Cell Research, 2019 - Elsevier
Integration-free induced pluripotent stem cell (iPSC) lines from the blood of healthy adult
individuals cells can serve as controls for drug development and disease modeling studies. …

An overview on promising somatic cell sources utilized for the efficient generation of induced pluripotent stem cells

A Ray, JM Joshi, PK Sundaravadivelu, K Raina… - Stem Cell Reviews and …, 2021 - Springer
… , urine cells, and peripheral blood mononuclear cells used for the generation of human iPSCs
… Therefore, various integration-free reprogramming approaches have been employed to …

An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

KK Haridhasapavalan, MP Borgohain, C Dey, B Saha… - Gene, 2019 - Elsevier
… This will reduce the number of transductions and the requirement of high viral titers for
reprogramming experiments, resulting in the generation of integration-free iPSCs. Therefore, use

[HTML][HTML] Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells using non-integrating vectors

W Chen, C Wang, ZX Yang, F Zhang, W Wen… - Communications …, 2023 - nature.com
… for reprogramming into induced pluripotent stem cells (iPSCs) 2,3 , since phlebotomy is …
integration-free human iMSCs, we reprogramed human PBMCs into iMSCs directly using

[HTML][HTML] The StemCellFactory: a modular system integration for automated generation and expansion of human induced pluripotent stem cells

A Elanzew, B Nießing, D Langendoerfer… - … in bioengineering and …, 2020 - frontiersin.org
… the automated generation of peripheral blood mononuclear cells for subsequent
reprogramming using the StemCellFactory (Elanzew, Breitkreuz, unpublished observations). …

[HTML][HTML] Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector

T Okumura, Y Horie, CY Lai, HT Lin, H Shoda… - Stem Cell Research & …, 2019 - Springer
induced pluripotent stem cells (iPSCs) is a powerful tool for elucidating the mechanisms
underlying disease pathogenesis and developing safe and effective … to obtain integration-free