[HTML][HTML] Studying membrane modulation mechanisms by electron cryo-tomography

D Zabeo, KM Davies - Current Opinion in Structural Biology, 2022 - Elsevier
… With the development of electron cryo-tomography and … Here, we review the different ways
electron cryo-tomography … In this review, we discuss how electron cryotomography (cryoET) is …

[HTML][HTML] Towards high-throughput in situ structural biology using electron cryotomography

J Böhning, TAM Bharat - Progress in biophysics and molecular biology, 2021 - Elsevier
Electron cryotomography is a rapidly evolving method for imaging macromolecules directly
… macromolecules using correlated light and electron microscopy and advancements made to …

Cellular electron cryo-tomography to study virus-host interactions

ERJ Quemin, EA Machala, B Vollmer… - Annual review of …, 2020 - annualreviews.org
Viruses are obligatory intracellular parasites that reprogram host cells upon infection to
produce viral progeny. Here, we review recent structural insights into virus-host interactions in …

A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0

J Zivanov, J Otón, Z Ke, A von Kügelgen, E Pyle, K Qu… - Elife, 2022 - elifesciences.org
We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

Rapid tilt-series acquisition for electron cryotomography

G Chreifi, S Chen, LA Metskas, M Kaplan… - Journal of structural …, 2019 - Elsevier
… We characterize the utility of both methods in real biological electron cryotomography
workflows. We identify opportunities for further improvements in hardware and software and …

[HTML][HTML] Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism

S Li, JJ Fernandez, WF Marshall, DA Agard - Elife, 2019 - elifesciences.org
… Using electron cryo-tomography (cryoET) we present the structure of procentrioles from
Chlamydomonas reinhardtii. We identified a set of non-tubulin components attached to the triplet …

In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography

M Kaplan, WJ Nicolas, W Zhao, SD Carter… - CryoEM: Methods and …, 2021 - Springer
Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact
macromolecular complexes while they are in their cellular milieu. Over the years, cryo-ET has …

Electron cryotomography of bacterial secretion systems

CM Oikonomou, GJ Jensen - Microbiology spectrum, 2019 - Am Soc Microbiol
… Before the advent of electron cryotomography (ECT), we knew an impressive amount about
what secretion systems are made of; genetics revealed components, biochemistry revealed …

[PDF][PDF] High-resolution structure determination using high-throughput electron cryo-tomography

HF Liu, Y Zhou, A Bartesaghi - Acta Crystallographica Section D …, 2022 - journals.iucr.org
… of tilt series per data set deposited in the Electron Microscopy Pilot Image Archive (EMPIAR) …
Our recently proposed beam-image shift electron cryo-tomography (BISECT) strategy …

[HTML][HTML] Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography

NB Gudimchuk, EV Ulyanov, E O'Toole… - Nature …, 2020 - nature.com
… Evidence from early electron cryomicroscopy revealed mostly blunt ends on growing MTs …
Our recent descriptions of MT tips by electron cryotomography (cryoET) in vivo and in vitro …