… The transplantation of MSCs into each of the tissues that originates from the ectoderm,
endoderm and mesoderm causes the cells to differentiate into the target tissue. The iPSCs have …

… of insulin-producing cells from human pluripotent stem cells (… are differentiated through the
stages of definitive endoderm (… to 3D culture for differentiation into EPs-3D and PECs-3D. b–i …

… differentiation of human pluripotent stem cells (hPSCs) into definitive endoderm (DE) represents
a key step towards somatic cells … and subsequently into insulin producing cells 24,47,48 …

… endoderm markers like FoxA2 and Sox-17 were measured by quantitative PCR. The maximum
levels of definitive endoderm markers were detected in the 3D … , using 3D culture system …

… the differentiation of cells into a definitive endoderm and … of a total induction period of 3 d.
Immunostaining revealed that … similarly induced differentiation into IPCs over 8 days in culture …

… β-cell cultures in vitro to increase the supply of these cells for … control the differentiation of
induced pluripotent stem cells (… parameters for efficient endoderm differentiation is definition …

… induced pluripotent stem cells (iPSCs) are a group of cells … A and Wnt3 is used to definitive
endoderm generation. As in the … of the 3D media culture on the survival and differentiation of …

… insulin-producing cells (IPCs) holds great promise for cell … of pancreatic development, including
definitive endoderm (stage 1), … Therefore, we hypothesized that the 3D induction method …

… ) to provide vector and 3D culture conditions for cells, and we … of differentiation of hDPSCs
into insulin-producing cells (IPCs… to differentiate into definitive endoderm-like cells, pancreatic …

… differentiation methodology for generating human pluripotent … added during the subsequent
3 d of endoderm induction. At … for HUES8 and two induced pluripotent stem cell (iPSC) lines …