… Higher butanol production was achieved by inactivating … analysis showed that butanol
formation flux increased up to 300% … clostridia for the direct use of cellulose for solvent production …

… to reinforce or construct a butyrate reassimilated route uncoupled … to direct carbon flow
toward butyryl-CoA, the n-butanol … We previously obtained an evolved strain with high butanol …

… different routes: a direct route with ALD and ADH and an indirect … be achieved with optimized
fermentation conditions and better … Further studies strengthening the C2 to C4 pathway in C. …

… Enhanced butanol production obtained by reinforcing the direct butanol-forming route in
Clostridium acetobutylicum [J]. mBio, 2012, 3(5): e00314-12. [46] BOND-WATTS BB, …

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… 6a) favored butanol production and significantly increased the butanol yield (from 50% to 72…

… by studying the seven genes involved in the butanol forming … (121 C for 15 min) Reinforced
Clostridium Medium (RCM). … facilitate the direct production of butanol through the routes of …

… cellulovorans DSM 743B to enhance butanol production. It was engineered via integrated
… The authors declare financial support was received for the research, authorship, and/or …

… Butanol production by Clostridium acetobutylicum is … The much-increased B/E ratio obtained
was due to the … S2), implying that the mutational effect can be either direct or indirect. We …

… than achieved using the strong P fdx promoter of the Clostridium … to play, both in enhancing
productivity and extending product … to recombination between the direct repeats that flank the …

… cells were cultured on reinforced Clostridium medium (RCM, … butanol and ABE productions
of 12.3 and 20.2 g/L obtained … gene adhE1 D485G in butanol-forming pathway, culture pH > …