Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine

R Datta, A Gillette, M Stefely… - Journal of Biomedical …, 2021 - spiedigitallibrary.org
FLIM is a powerful molecular imaging technique that is widely used in biology and medicine
major advances in FLIM instrumentation, analysis, and biological and clinical applications …

FLIM as a promising tool for cancer diagnosis and treatment monitoring

Y Ouyang, Y Liu, ZM Wang, Z Liu, M Wu - Nano-Micro Letters, 2021 - Springer
… Fluorescence lifetime imaging microscopy (FLIM) has been … FLIM optimization, offering
more opportunities for medical … -related FLIM applications and the potential of FLIM for future …

Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications

H Ranawat, S Pal, N Mazumder - Biomedical Engineering Letters, 2019 - Springer
FLIM promises to provide greater biochemical and medical … of FLIM on a multiphoton
laser scanning microscope is … Additionally, FLIM set-ups predominantly have the ability to …

[PDF][PDF] Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy (FLIM)

J Qu, L Liu - Journal of Innovative Optical Health Sciences, 2019 - researching.cn
FLIM imaging. As the application of FLIM in biological eld progresses, new requirements for
microscopy (FLIM) monitors tumor cell death triggered by photothermal therapy with MoS2 …

Research of Pulmonary Fibrosis Lesions Based on FLIM and SHG Imaging Microscopy

W Li, X Li, C Zhang, H Wang, Y Zhu, Y Wang… - Analytical …, 2024 - ACS Publications
… Two-photon fluorescence lifetime microscopy (TP-FLIM) is a powerful quantitative imaging …
biological samples through a combination of intensity and lifetime imaging. Because TP-FLIM

[PDF][PDF] Applications of FLIM in regenerative medicine

A Kashina, D Kuznetsova, V Elagin, V Dudenkova… - 2021 - scholar.archive.org
… lifetime imaging microscopy (FLIM), enabling live quantitative multiparametric analysis, is
an emerging bioimaging approach in tissue engineering and regenerative medicine. FLIM in …

Clinical in vivo multiphoton FLIM tomography

K König - Methods and applications in fluorescence, 2020 - iopscience.iop.org
… with this unique first confocal ultrashort laser FLIM microscope at that time in 1988/1989 even
it … After obtaining the CE certificate as medical product, two 5D spectral FLIM tomographs …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
… -based fluorescence microscopy methods. Then, we review FLIM instrumentation and FLIM
analysis … systems use electronics with short dead times to increase frame rates for medical

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding

M Barroso, MG Monaghan, R Niesner… - Advanced Drug Delivery …, 2023 - Elsevier
… Thus, fluorescence lifetime imaging microscopy (FLIM) and … , permitting its application in
microscopy as well as in small … approaches to construct a wide variety of FLIM microscopes. …

Estimation of the mitochondrial membrane potential using fluorescence lifetime imaging microscopy

IA Okkelman, DB Papkovsky, RI Dmitriev - Cytometry Part A, 2020 - Wiley Online Library
… Two-photon FLIM microscopy of intestinal organoids was performed on a “Dive” Falcon
FLIM TCS SP8 DM6B-Z-CFS multiphoton system, equipped with HC PL IRAPO 25×/1.00 water …