Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
technique has a large influence on the performance of a FLIM system. Basically, fluorescence
lifetime imaging … wide-field detection, or by scanning the sample with a focused laser beam …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
… Analytical tools for fluorescence lifetime estimations, image segmentation, and heterogeneity
analysis are introduced in this section. Many popular methods are highlighted and …

Fast fit-free analysis of fluorescence lifetime imaging via deep learning

JT Smith, R Yao, N Sinsuebphon… - Proceedings of the …, 2019 - National Acad Sciences
image formation that is based on deep learning (DL) to quantify fluorescence decays
simultaneously over a whole image … architecture, named fluorescence lifetime imaging network …

Review of fluorescence lifetime imaging microscopy (FLIM) data analysis using machine learning

M Adhikari, R Houhou, J Hniopek… - Journal of Experimental …, 2023 - mdpi.com
techniques have been developed, such as the Net-FLICS network for fluorescence lifetime
imaging using compressive sensing data and the 3D CNN-based FLI-Net network for lifetime

Spatial resolution improved fluorescence lifetime imaging via deep learning

D Xiao, Z Zang, W Xie, N Sapermsap, Y Chen… - Optics …, 2022 - opg.optica.org
… to rapidly obtain HR FLIM images from raw image data … method combines DL-based
fluorescence lifetime determination and single image resolution enhancement (DL-SIRE) techniques

Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

N Inada, N Fukuda, T Hayashi, S Uchiyama - Nature protocols, 2019 - nature.com
… is recognized as the method that most accurately measures fluorescence lifetime 66,67 . …
fluorescent molecules with multiple lifetime components for which both the fluorescence lifetime

Net-FLICS: fast quantitative wide-field fluorescence lifetime imaging with compressed sensing–a deep learning approach

R Yao, M Ochoa, P Yan, X Intes - Light: science & applications, 2019 - nature.com
… CNN named Net-FLICS (fluorescence lifetime imaging with compressed sensing), which
enables direct DL-based fluorescence intensity and lifetime imaging from time-resolved single-…

Luminescence lifetime imaging using a cellphone camera with an electronic rolling shutter

B Xiong, Q Fang - Optics Letters, 2019 - opg.optica.org
method to achieve luminescence lifetime imaging. Here we report a low-cost luminescence
lifetime imaging … The luminescence lifetime imaging is validated by mapping the interaction …

FLImBrush: dynamic visualization of intraoperative free-hand fiber-based fluorescence lifetime imaging

M Marsden, T Fukazawa, YC Deng… - Biomedical Optics …, 2020 - opg.optica.org
… Here we report FLImBrush as a robust method for the … fluorescence lifetime imaging (FLIm).
FLImBrush builds upon an existing method while employing deep learning-based image

Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy

JE Sorrells, RR Iyer, L Yang, AJ Bower… - Biomedical optics …, 2021 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) is an optical imaging technique that
characterizes the fluorescence profile of each pixel within an image by the average time between …