… identified from Candida glabrata through genome hunting, … /reductases (SDRs) were cloned
from the genomic DNA of C. … 3A–C, CPMK is situated in the active center with the carbonyl …

… that the C3 carbonyl oxygen of KPL formed hydrogen bond with the side chains of Tyr63,
and the α-amino group of Thr27 also formed a hydrogen bond with C2 carbonyl oxygen (Fig. 7d…

… Tremendous efforts have been made in exploring efficient strategies for the synthesis of …
)ethanols, genome hunting strategy was adopted in screening for carbonyl reductases from …

… So far, only a few examples of robust carbonyl reductases … is a carbonyl reductase CgKR2
from Candida glabrata, which … harbouring GoCR or its mutant gene were transformed into E. …

… from Debaryomyces hansenii and Candida glabrata with high … (BaSDR1) was discovered
by the genome hunting of Bacillus … Carbonyl reductase ChKRED03 obtained through gene …

… Therefore, new and efficient methods, such as genome hunting and database … gene-mining
strategy, which uses known reductase sequences as probe to BLAST in public gene data or …

… , which is an effective method for preparing nanopolymer-multi-… ) as a backbone to immobilise
carbonyl reductase and glucose … Extraction of the gene encoding Candida parapsilosis …

… Enzymatic asymmetric reduction by carbonyl reductases or … marxianus CBS4857 by
genome mining with the sequence of … roles in the catalytic efficiency toward α-chloroacetophenone, …

… effective protocol was developed for the directed resurrection of ancestral enzymes. Employing
genome … The T15 50 and T m values of A64 were 57.5 C and 61.7 C, significantly higher …

… as alcohol dehydrogenases or carbonyl reductases. KREDbelongs to … Among these, CglCPR
from Candida glabrata exhibited a … of gene databases, new enzyme mining based on gene …