… efficiency compared to single pulse electroporation. Other cell growth and pretreatment …
by the inability to transform the cells with high efficiency. The typical method for transferring …

… and biolistic bombardment to normal or permeabilized cells (Noda et al. 2017… high efficiency
nuclear transformation of N. limnetica by electroporation directly to wild-type cells pretreated …

… “Scrutinizing a Lactococcus lactis strain with enhanced capacity for extracellular electron
transfer reveals a unique role for a novel type II NADH dehydrogenase”. Cell metabolism …

… lactis strains by electroporation described by Holo and Nes [… amplitude values of ultrasound
pretreatment were chosen as 50. … lactis cells and pLEB124 vector was constructed harboring …

… We compared the performance of NpB cells collected at 3, 9, … higher efficiency and
accuracy in signal acquisition compared to the use of immunochemical compounds. Pre-treatment …

… growth, PW was pretreated with HCl (20 mL 32% HCl in 1 L PW) for 10 … coli were transformed
using electroporation. … Our results demonstrated that LacDia was an efficient cell factory for …

… Lactococcus lactis is an important industrial microorganism … the editing efficiency up to 17,600
CFU per 10 8 cells (~2 × 10 −… ,31], allow for a high efficiency of the gene knock-out process …

… an electroporation method was developed to realize the introduction of exogenous DNA into
C. crustorum with high efficiency… cells and subsequently into MN047 cells by electroporation …

… , 1 ml of SGM17 medium was poured on the cells immediately. The transferred bacteria were
… electroporation process of recombinant pBU003 plasmids to MG1363 Lactococcus lactis …

… cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2
… lactis cells were pretreated for 60 min with 4 mg lysozyme/mL in THMS buffer (30 mmol/…