Multiple pulse electroporation of lactic acid bacteria Lactococcus lactis and Lactobacillus casei
DL Welker, BM Coburn, JH McClatchy… - Journal of microbiological …, 2019 - Elsevier
… efficiency compared to single pulse electroporation. Other cell growth and pretreatment …
by the inability to transform the cells with high efficiency. The typical method for transferring …
by the inability to transform the cells with high efficiency. The typical method for transferring …
High efficiency transformation by electroporation of the freshwater alga Nannochloropsis limnetica
Y Chen, H Hu - World Journal of Microbiology and Biotechnology, 2019 - Springer
… and biolistic bombardment to normal or permeabilized cells (Noda et al. 2017… high efficiency
nuclear transformation of N. limnetica by electroporation directly to wild-type cells pretreated …
nuclear transformation of N. limnetica by electroporation directly to wild-type cells pretreated …
Extracellular Electron Transfer of Lactococcus lactis: Fundamentals and Applications
L Gu - 2023 - orbit.dtu.dk
… “Scrutinizing a Lactococcus lactis strain with enhanced capacity for extracellular electron
transfer reveals a unique role for a novel type II NADH dehydrogenase”. Cell metabolism …
transfer reveals a unique role for a novel type II NADH dehydrogenase”. Cell metabolism …
Extracellular recombinant production of 4, 6 and 4, 3 α-glucanotransferases in Lactococcus lactis
… lactis strains by electroporation described by Holo and Nes [… amplitude values of ultrasound
pretreatment were chosen as 50. … lactis cells and pLEB124 vector was constructed harboring …
pretreatment were chosen as 50. … lactis cells and pLEB124 vector was constructed harboring …
[HTML][HTML] Double-Labeling Method for Visualization and Quantification of Membrane-Associated Proteins in Lactococcus lactis
MN Hoang, C Peterbauer - International Journal of Molecular Sciences, 2023 - mdpi.com
… We compared the performance of NpB cells collected at 3, 9, … higher efficiency and
accuracy in signal acquisition compared to the use of immunochemical compounds. Pre-treatment …
accuracy in signal acquisition compared to the use of immunochemical compounds. Pre-treatment …
[HTML][HTML] Food grade microbial synthesis of the butter aroma compound butanedione using engineered and non-engineered Lactococcus lactis
JM Liu, L Chen, PR Jensen, C Solem - Metabolic Engineering, 2021 - Elsevier
… growth, PW was pretreated with HCl (20 mL 32% HCl in 1 L PW) for 10 … coli were transformed
using electroporation. … Our results demonstrated that LacDia was an efficient cell factory for …
using electroporation. … Our results demonstrated that LacDia was an efficient cell factory for …
[HTML][HTML] New effective method of Lactococcus genome editing using guide RNA-directed transposition
PY Pechenov, DA Garagulya, DS Stanovov… - International Journal of …, 2022 - mdpi.com
… Lactococcus lactis is an important industrial microorganism … the editing efficiency up to 17,600
CFU per 10 8 cells (~2 × 10 −… ,31], allow for a high efficiency of the gene knock-out process …
CFU per 10 8 cells (~2 × 10 −… ,31], allow for a high efficiency of the gene knock-out process …
Development of an electroporation method and expression patterns of bacteriocin-encoding genes in Companilactobacillus crustorum MN047
P Wang, T Wang, M Ismael, X Wang, Y Yi, X Lü - Food Bioscience, 2021 - Elsevier
… an electroporation method was developed to realize the introduction of exogenous DNA into
C. crustorum with high efficiency… cells and subsequently into MN047 cells by electroporation …
C. crustorum with high efficiency… cells and subsequently into MN047 cells by electroporation …
Signal Peptide Optimization, Cloning, Expression and Characterization of Ce16B Cellobiohydrolase in Lactococcus lactis
MH Jahandar, H Sarhadi, A Tanhaeian - International Journal of Peptide …, 2020 - Springer
… , 1 ml of SGM17 medium was poured on the cells immediately. The transferred bacteria were
… electroporation process of recombinant pBU003 plasmids to MG1363 Lactococcus lactis …
… electroporation process of recombinant pBU003 plasmids to MG1363 Lactococcus lactis …
Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine …
M Rezaei, M Rabbani Khorasgani… - Canadian journal of …, 2020 - cdnsciencepub.com
… cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2
… lactis cells were pretreated for 60 min with 4 mg lysozyme/mL in THMS buffer (30 mmol/…
… lactis cells were pretreated for 60 min with 4 mg lysozyme/mL in THMS buffer (30 mmol/…