… the validation of reference genes to identify the most suitable internal control for normalization
of qRT-PCR data from different … of candidate reference genes across all the experimental …

… of qRT-PCR to a large extend. Therefore, the present study was designed to identify and
validate the suitable reference genes … the candidate reference genes before each experiment. …

… to identify the most appropriate reference genes for qRT-PCR of miRNAs and mRNAs in
different tissues at … experimental condition in different tissues or species [5]. However, no single …

… in different experimental tissues, we further carried out comprehensive stability analysis for
all 10 candidate reference genes. … , was used for reference gene validation in this study. As …

… This tool has been widely used and validated by many … screening reference genes from
extensive experimental datasets… of each gene may be the most difficult part because of qRT-PCR …

… data validation step is still required using the real-time qRT-PCR … set of miRNAs that can be
tested in each experiment [26,59]. … step-by-step experimental procedure for RG identification. …

… The qRT-PCR is the most accurate and sensitive method to … The overall experimental
setup was accordingly fast and … the possibility of evaluation of 19 candidate reference genes. …

… experimental conditions, species or tissues, reference genes … on validating a set of commonly
used reference genes. … the identification of ideal RGs can be based on in silico analysis…

… according to different species and experimental conditions [… study is to identify appropriate
reference genes for qRT-PCR … , we validated the expression levels of the key genes related …

… housekeeping genes might not be suitable for all materials under different experimental …
conducted to identify reliable RGs for various plant materials at different developmental stages. …