… These findings illustrate the power of in vivo transposon mutagenesis when coupled with …
of insertion mutants can be generated and screened by combining in vivo transposition with …

… For the supply of Candida glabrata mutants used in this work, I … efflux pump overexpressed
in azole resistant strains. As far as … In vivo studies of emergent drug resistance mechanisms …

… glabrata has primarily been due to mutations in the CgFKS1 … For in vivo virulence analysis,
we infected BALB/c mice with … wt and azole-resistant Cgfpr3Δ4Δ mutant strains. Notably, the …

… Examining how these mutations have spread through the … of drug, by combining data from
in vivo screening, genetic interactions, … However, because only 50% of azole-resistant clinical …

… lost the CRISPRa plasmid are selected after two rounds of culture in YPD at 37C. P value >
… , our results suggest that even if mutations lead to a weak increased transcriptional activity (…

… resistance by isolating azole-resistant S. cerevisiae … To confirm that these were petite
mutants, all clones were cultured … efficacy of our selection protocols. However, many additional …

… Furthermore, in vivo experiments also confirmed … , rapid drug efflux accomplished by
select transporters stands … glabrata IPT1 mutant. Even with a high level of ergosterol in …

… Azole-resistant isolates from candidemia patients have been observed at low frequency for
… GOF mutation fitness has been investigated both in vitro and in vivo for C. albicans. Strains …

… have been shown to be “petite” ie, with mitochondrial defects … therapy by undergoing mutation
either in vitro or in vivo (101)… most persistent causes of azole-resistant are mutations in the …

… Species including as Candida albicans, Candida glabrata, Candida dubliniensis and …
selected were those smaller than the control (without inhibitor). The identity of the petite mutants …