RecAindependent recombination: Dependence on the Escherichia coli RarA protein

K Jain, EA Wood, ZJ Romero… - Molecular microbiology, 2021 - Wiley Online Library
… Whereas most detectable homologous recombination events … (b) Plasmids were isolated
from Tet R recombinants from the … Increases in RecA and RarA-independent recombination

RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation

M Skutel, D Yanovskaya, A Demkina… - Nucleic Acids …, 2024 - academic.oup.com
… , RecA-independent, homologous recombination pathway that … Escherichia coli (24,25).
Depending on the irradiation dose, … DNA, which should increase the frequency of recombination. …

RecO impedes RecG-SSB binding to impair the strand annealing recombination pathway in E.coli

X Pan, L Yang, N Jiang, X Chen, B Li, X Yan, Y Dou… - bioRxiv, 2019 - biorxiv.org
… of SSB in vivo, and the partial increase in UV resistance by … in a RecA-independent repair
on DNA double strand breaks … For example, the RecO protein isolated from both E.coli and …

DNA damage-signaling, homologous recombination and genetic mutation induced by 5-azacytidine and DNA-protein crosslinks in Escherichia coli

JA Klaric, DJ Glass, EL Perr, AD Reuven… - … of Mutagenesis, 2021 - Elsevier
… We used a chromosomal lacZ mutational reporters specific for G to C … large increase in
mutation frequency by Pol IV when induced. Induction of Pol II caused about a 3-fold increase in …

Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli

T Ding, C Huang, Z Liang, X Ma, N Wang… - Microbial Cell Factories, 2020 - Springer
… in most types of homologous recombination events, but not in … RecA-independent
recombination is enhanced by mutations … Moreover, even if isolation of plasmid containing repeat …

Modulation of RecFORQ-and RecA-mediated homologous recombination in Escherichia coli by isoforms of translation initiation factor IF2

J Mallikarjun, L SaiSree, P Himabindu… - Journal of …, 2022 - Am Soc Microbiol
… for chromosomal replication, as well as DNA damage repair in all life forms. In Escherichia
coli… , indicate that the rho mutant does exhibit a moderate increase in HR frequency (Fig. S1E). …

Enhancing the Protein Engineering Toolbox: In Vivo Recombination in E. Coli for Multi-Site-Directed Mutagenesis

DHF Costa - 2021 - search.proquest.com
… via an in vitro homologous recombination with a manufacturer … full potential of this
RecA-independent pathway as a molecular … template DNA which had been isolated from a dam+ …

The pathway of recombining short homologous ends in Escherichia coli revealed by the genetic study

Y Yang, T Wang, Q Yu, H Liu, L Xun… - Molecular …, 2021 - Wiley Online Library
RecA-independent homologous recombination also occurs … without homologous arms to a
gradual increase from 12 to 30 … with DpnI and purified with the same Gel Extraction Kit. This …

[HTML][HTML] Natural transformation in Deinococcus radiodurans: a genetic analysis reveals the major roles of DprA, DdrB, RecA, RecF, and RecO proteins

S Ithurbide, G Coste, J Lisboa, N Eugénie… - Frontiers in …, 2020 - frontiersin.org
DNA, will favor the loading of RecA onto the genomic DNA to initiate homologous recombination
with the genome of the … (IIIb) In a RecA independent manner, DdrB, via its single strand …

Identification of recG genetic interactions in Escherichia coli by transposon sequencing

NJ Bonde, EA Wood, KS Myers, M Place… - Journal of …, 2023 - Am Soc Microbiol
… branched recombination intermediates during DNA repair … recG ruv mutant strains improves
when mutations are made … from each culture (Promega Wizard gDNA extraction kit) and …