Background:‘Do not resuscitate (DNR) order’is a decision taken by the patient, authorized person (s) or competent healthcare professionals in the end stages of patient’s life regarding not to perform CPR on a patient during cardiac arrest or pulmonary arrest (cardio-respiratory arrest) Aim: To access the knowledge, attitude and practice of general public residing in Aseer, Saudi Arabiaabout ‘DNR’in order to find out the barriers and obstacles in addressing DNR status of the patients. Methodology: Cross-sectional, KAP questionnaire based survey was conducted intertiary health care center of King Khalid University and associated hospital, Saudi Arabia. The structured questionnaire consisted of total 15 questions related to ‘DNR orders’ and 05 questions regarding general demographic data. Thensoft copy of ‘DNR survey questionnaire form’was send randomly to 3324 subjects (both male and female) by various social media applications thatincluded Google survey link with questionnaire. After the completion of study, the data was collected, tabulated and subjected to descriptive analysis using IBM SPSS version 20.0 software. Results: Overall response rateof 98.10% was noted after distributing 3324 ‘DNR survey questionnaire form’withmaximum respondents’ belong to 21-30 year age group. 62.38% of participants had never heard about DNR. 63.84% respondents’ are of opinion that age is not the only criteria for DNR. 34.40% were not aware of the indications of DNR. Most of the respondents’ believed that DNR decision should be taken by medical team from hospital side that too after discussion with patient. Conclusion: low education level regarding medical care at the end-of-life, lack of understanding and inadequate training among general public were the main barriers and obstacles for initiation and completion of DNR orders in Aseer, Saudi Arabia.