Proper hand washing technique is very easy to learn and can significantly reduce the infectious diseases among children. This descriptive crosssectional school-based study was conducted in Ombadda locality Khartoum State Sudan, with an objective to study the knowledge, attitude and practices among basic schools pupils towards hand washing. A sample size of 400 pupils was determined using statistical formula, data were collected using interview and observation, and a pre-prepared and pre-tested questionnaire, and data were analyzed using SPSS. The majority of the pupils 95% knew the proper way of hand washing with soap and water, 96% knew the appropriate times for hand washing, sixty five percent of the pupils knew the benefits of hand washing and 88.2% knew diseases related to the practice of not washing hands. The twenty five percent of the pupil’s information about hand washing is from family members, 94.8% had positive attitude towards hand washing with soap and water, 70.3% of the pupils practice hand washing with soap and water at appropriate times, 39.5% practice hand washing in time ranged.