It's what's on the inside that counts: Techniques for investigating the uptake and recycling of nanoparticles and proteins in cells

LI FitzGerald, APR Johnston - Journal of Colloid and Interface Science, 2021 - Elsevier
… Finally, we highlight the advantages of using internalization … 6) can distinguish between
surface bound and internalised … The amount of information that can be extracted from the data …

Physicochemical tools for studying virus interactions with targeted cell membranes in a molecular and spatiotemporally resolved context

M Bally, S Block, F Höök, G Larson, N Parveen… - Analytical and …, 2021 - Springer
… efficiently probed using total internal reflection fluorescence (… it possible to visualize
surface-bound fluorescently labeled … , it is possible to distinguish binding events caused by viruses …

A specific hybridisation internalisation probe (SHIP) enables precise live-cell and super-resolution imaging of internalized cargo

S Hernández-Pérez, PK Mattila - Scientific Reports, 2022 - nature.com
… to unambiguously distinguish the internalised BCR-antigen complexes from the surface-bound
In order to push the speed limits of image acquisition, we turned to deep learning-based …

Biomedical analysis of exosomes using biosensing methods: recent progress

HK Kordasht, M Hasanzadeh - Analytical Methods, 2020 - pubs.rsc.org
… can be used to identify various types of external and internal … of several advantages, including
the lack of need for a separate … -positive (as the surface-bound mass) exosomes was 6.3 ± …

An exploration of aptamer internalization mechanisms and their applications in drug delivery

LY Wan, WF Yuan, WB Ai, YW Ai, JJ Wang… - Expert opinion on …, 2019 - Taylor & Francis
… White used a cell-SELEX approach to identify cell-internalizing … , proteinases are also used
to remove cell surface-bound … These methods have their own advantages and disadvantages

CRISPR-Cas9 screens identify regulators of antibody–drug conjugate toxicity

CK Tsui, RM Barfield, CR Fischer, DW Morgens… - Nature chemical …, 2019 - nature.com
… of ADC used in the screens, both of which could limit the … To identify which steps of ADC
internalization and processing are … 2.8) with shaking at 4 C for 10 min to remove surface-bound

CMTM4 regulates angiogenesis by promoting cell surface recycling of VE-cadherin to endothelial adherens junctions

I Chrifi, L Louzao-Martinez, MM Brandt, CGM Van Dijk… - Angiogenesis, 2019 - Springer
… To identify novel regulators of … Surface bound VE-cadherin antibodies were removed
by an acid-wash, before proceeding with immunostaining for visualization of internalized VE-…

Modelling the impact of nucleolin expression level on the activity of F3 peptide-targeted pH-sensitive pegylated liposomes containing doxorubicin

R Lopes, K Shi, NA Fonseca, A Gama… - Drug Delivery and …, 2022 - Springer
… levels at the cell membrane, binding and internalization of pH-… C, cell surface-bound liposomes
were removed with low pH … 4d), a difference reflecting the spatial constraints of liposome …

Identifying molecular tags selectively retained on the surface of brain endothelial cells to generate artificial targets for therapy delivery

GM Porro, I Lorandi, X Liu, K Kataoka… - Fluids and Barriers of …, 2023 - Springer
… we aimed to identify peptides with a long internalization half-life … , would have clear
advantages over current RMT strategies. … of avidin by cell-surface bound CFAG. We attribute this …

Fluorescent carbon dots for selective labeling of subcellular organelles

B Unnikrishnan, RS Wu, SC Wei, CC Huang… - Acs Omega, 2020 - ACS Publications
… (26) The surface-bound PEI enhances the fluorescence … limits the applications of organic
dyes in long durations of … due to their differences in surface chemistry, cell internalization, and/…