Surgical preparations, labeling strategies, and optical techniques for cell-resolved, in vivo imaging in the mouse spinal cord

YT Cheng, KM Lett, CB Schaffer - Experimental neurology, 2019 - Elsevier
… excited fluorescence microscopy, are largely limited to imaging … toxicity in living animals;
Third, the use of two-photon excited … In studies of neural structure and dynamics in the brain, the …

[HTML][HTML] Engineering a cell-hydrogel-fibre composite to mimic the structure and function of the tendon synovial sheath

A Imere, C Ligorio, M O'Brien, JKF Wong, M Domingos… - Acta Biomaterialia, 2021 - Elsevier
… for imaging. Fluorescence microscopy was used for observations using an Eclipse 50i … ,
the number of live and dead cells was obtained from the greyscale images of the green and red …

[HTML][HTML] High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

L Streich, JC Boffi, L Wang, K Alhalaseh, M Barbieri… - Nature …, 2021 - nature.com
… of our 3P microscope by imaging neuronal structure and third… (EGFP, enhanced green
fluorescent protein) mouse through a … among highly scattering myelinated fiber tracts deep within …

[HTML][HTML] Removing striping artifacts in light-sheet fluorescence microscopy: a review

P Ricci, V Gavryusev, C Müllenbroich, L Turrini… - Progress in biophysics …, 2022 - Elsevier
… , they proved their novelty by imaging living zebrafish embryos avoiding unwanted speckle
… light beam by implementing a supercontinuum fibre laser with large emission bandwidth and, …

Exceeding the limits of 3D fluorescence microscopy using a dual-stage-processing network

H Zhang, Y Zhao, C Fang, G Li, M Zhang, YH Zhang… - Optica, 2020 - opg.optica.org
… {mm}$ with diverse neuronal structures distributed across many … live model organisms by
performing the imaging of neuronalFiber-coupled lasers with four excitation wavelengths (405, …

Pushing the frontiers: tools for monitoring neurotransmitters and neuromodulators

Z Wu, D Lin, Y Li - Nature Reviews Neuroscience, 2022 - nature.com
fibre photometry, wide-field epifluorescence imaging, light-sheet fluorescence imaging,
multiphoton imaging … body in a live fly measured using two-photon microscopy. To express GRAB …

Fluorogenic and cell‐permeable rhodamine dyes for high‐contrast livecell protein labeling in bioimaging and biosensing

D Si, Q Li, Y Bao, J Zhang… - Angewandte Chemie …, 2023 - Wiley Online Library
… of fluorescence microscopy techniques has opened up new opportunities for visualizing
proteins and unraveling their functions in living … to the structural features of the chromatin fiber. …

[HTML][HTML] A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging

L Wang, C Wu, W Peng, Z Zhou, J Zeng, X Li… - Nature …, 2022 - nature.com
… cAMP dynamics in living animals, we performed head-fixed two-photon imaging in the …
into the NAc and measured fluorescence signals using fiber photometry while the mice were …

[HTML][HTML] Polarization modulation with optical lock-in detection reveals universal fluorescence anisotropy of subcellular structures in live cells

M Guan, M Wang, K Zhanghao, X Zhang, M Li… - Light: Science & …, 2022 - nature.com
structural changes, interactions, and function of subcellular. Over the past decades, super-resolution
fluorescent microscopy brought the vision of the structure … actin fiber bundles, with …

Using confocal imaging approaches to understand the structure and function of osteocytes and the lacunocanalicular network

SL Dallas, DS Moore - Bone, 2020 - Elsevier
… cortex synapses in a human brain, with an estimated nerve fiber length of … fluorescence
microscopes are increasingly being used for live imaging applications, particularly intravital (live