The Non-native Disulfide-Bond-Containing Landscape Orthogonal to the Oxidative Protein-Folding Trajectory: A Necessary Evil?
M Narayan - The Journal of Physical Chemistry B, 2022 - ACS Publications
… native disulfide bonds; ie, only native disulfide bonds provoke folding to form native-like/native …
Analytical limitations have prevented mapping the distribution of disulfide bonds in the 3S …
Analytical limitations have prevented mapping the distribution of disulfide bonds in the 3S …
Revisiting the formation of a native disulfide bond: consequences for protein regeneration and beyond
M Narayan - Molecules, 2020 - mdpi.com
… detailed map of the folding pathway and the folding kinetics can be constructed (Scheme
2). … -containing proteins constitute approximately 15% of the human proteome [113,114]. …
2). … -containing proteins constitute approximately 15% of the human proteome [113,114]. …
ProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping
… and disulfide bond mapping in mAb. The results demonstrate that ProAlanase is highly suitable
for proteomics … is efficient in disulfide bond mapping, showing high coverage of disulfide-…
for proteomics … is efficient in disulfide bond mapping, showing high coverage of disulfide-…
Probing the Architecture of Multisubunit Protein Complexes with In-line Disulfide Reduction and Native MS Analysis
DG Ivanov, K Cheung, IA Kaltashov - Analytical Chemistry, 2024 - ACS Publications
… dissociation of the disulfide bonds, their application to mapping the noncovalent interactions
… -containing proteins account for about a third of the human proteome, (28) and their fraction …
… -containing proteins account for about a third of the human proteome, (28) and their fraction …
Streamlining the characterization of disulfide bond shuffling and protein degradation in IgG1 biopharmaceuticals under native and stressed conditions
J Coghlan, A Benet, P Kumaran, M Ford… - … in Bioengineering and …, 2022 - frontiersin.org
… disulfide bonds, we reported the disulfide bond data as the XIC sum contribution of all shuffled
disulfide bonds … of all (shuffled and expected) detected disulfide bonds. For the trisulfide …
disulfide bonds … of all (shuffled and expected) detected disulfide bonds. For the trisulfide …
A novel proteomic approach for the identification and relative quantification of disulfide-bridges in the human milk proteome
… the disulfide bridges present in the human milk proteome by a … relative quantification of the
disulfide bridges. Human milk from six … Mapping of disulfide bridges onto the protein structures …
disulfide bridges. Human milk from six … Mapping of disulfide bridges onto the protein structures …
Top-down mass spectrometry and assigning internal fragments for determining disulfide bond positions in proteins
B Wei, MA Zenaidee, C Lantz, BJ Williams, S Totten… - Analyst, 2023 - pubs.rsc.org
Disulfide bonds in proteins have a substantial impact on protein structure, stability, and biological
activity. Localizing disulfide bonds is … for disulfide-intact proteins, can access disulfide …
activity. Localizing disulfide bonds is … for disulfide-intact proteins, can access disulfide …
Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine
… Here, we describe a robust and rapid SS mapping approach that utilizes readily available
reagents, instruments, and software; it can be easily implemented in any analytical core …
reagents, instruments, and software; it can be easily implemented in any analytical core …
A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides
… A number of studies further validated a search engine by mapping the cross-linked …
cross-linker as well as native disulfide bonds, efficiently and effectively at a proteome scale. …
cross-linker as well as native disulfide bonds, efficiently and effectively at a proteome scale. …
Understanding unfolding and refolding of the antibody fragments (Fab). II. Mapping intra and inter-chain disulfide bonds using mass spectrometry
… This investigation is focused on mapping the intra, and inter-chain disulfide bonds in the in …
two intra-chain disulfide bonds on each subunit and a single inter-chain disulfide linkage. rHu …
two intra-chain disulfide bonds on each subunit and a single inter-chain disulfide linkage. rHu …