A metabolic labeling method detects m6A transcriptome-wide at single base resolution
X Shu, J Cao, M Cheng, S Xiang, M Gao, T Li… - Nature chemical …, 2020 - nature.com
… immunoprecipitation (IP)-based sequencing method (named m 6 … cellular metabolic a 6
A-labeled RNAs. During our search, we … , we speculated that m 6 A mRNA targets with a fast …
A-labeled RNAs. During our search, we … , we speculated that m 6 A mRNA targets with a fast …
Methods for RNA modification mapping using deep sequencing: established and new emerging technologies
… method was mostly applied to trace the incorporation of s 4 U nucleoside into nascent RNA
in metabolic labeling … -diol of a normal ribose is rapidly oxidized and cleaved, thus making it …
in metabolic labeling … -diol of a normal ribose is rapidly oxidized and cleaved, thus making it …
RNA sequencing by direct tagmentation of RNA/DNA hybrids
L Di, Y Fu, Y Sun, J Li, L Liu, J Yao… - Proceedings of the …, 2020 - National Acad Sciences
… Active residues in the RNHL domains are labeled in bright … tag RNA/DNA heteroduplexes
and, therefore, we have developed a quick RNA amplification and library preparation method …
and, therefore, we have developed a quick RNA amplification and library preparation method …
Reading canonical and modified nucleobases in 16S ribosomal RNA using nanopore native RNA sequencing
… labelled DNA nucleotides [8,9]. They share some of the benefits of nanopore native RNA
sequencing … Because nanopore data are collected in real-time, we examined how rapidly E. coli …
sequencing … Because nanopore data are collected in real-time, we examined how rapidly E. coli …
Mapping the epigenetic modifications of DNA and RNA
… rapidly evolving field. We focus on high-throughput detection … -based and chemical labeling
sequencing methods have been … a basic site at m 7 G positions, which can be further labeled …
sequencing methods have been … a basic site at m 7 G positions, which can be further labeled …
Recent advances in the detection of base modifications using the Nanopore sequencer
L Xu, M Seki - Journal of human genetics, 2020 - nature.com
… DNA and RNA modifications have important functions, … Existing methods based on short-read
sequencing for the detection of … Nascent RNAs are labeled by pulse labeling using base …
sequencing for the detection of … Nascent RNAs are labeled by pulse labeling using base …
Covalent labeling of nucleic acids
N Klöcker, FP Weissenboeck… - Chemical Society Reviews, 2020 - pubs.rsc.org
… Depending on the approach, site-, sequence- and cell-specificity can be achieved. DNA and
RNA labeling are rapidly developing fields that bring together multiple areas of research: on …
RNA labeling are rapidly developing fields that bring together multiple areas of research: on …
Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors
… to the sequence similarity to the on-target site. It is very fast and easy for researchers to
select the top dozen predicted sites and test them by targeted deep sequencing to figure out …
select the top dozen predicted sites and test them by targeted deep sequencing to figure out …
tailfindr: alignment-free poly (A) length measurement for Oxford Nanopore RNA and DNA sequencing
… (E) Vertical density plots of poly(A) length estimation from tailfindr (light green) and Nanopolish
(turquoise) on in vitro transcribed eGFP-RNA with poly(A) length of 40 or 150 nt (labeled …
(turquoise) on in vitro transcribed eGFP-RNA with poly(A) length of 40 or 150 nt (labeled …
SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues
… of multiplexing, multiple-round combinatorial labeling allows a … We use formamide to rapidly
destabilize short fluorescent … simple method to robustly detect RNA and DNA sequences in …
destabilize short fluorescent … simple method to robustly detect RNA and DNA sequences in …