Multiplex Digital Methylation‐Specific PCR for Noninvasive Screening of Lung Cancer
… This paper describes development and validation of the first multiplex digital methylation-specific
PCR (mdMSP) platform for simultaneous analysis of four methylation biomarkers for …
PCR (mdMSP) platform for simultaneous analysis of four methylation biomarkers for …
[HTML][HTML] Pyrosequencing versus methylation-specific PCR for assessment of MGMT methylation in tumor and blood samples of glioblastoma patients
A Estival, C Sanz, JL Ramirez, JM Velarde… - Scientific Reports, 2019 - nature.com
… Paired blood and tissue samples were assessed by methylation-specific PCR (MSP) and
pyrosequencing (PYR). After establishing the minimum PYR cut-off that could yield a significant …
pyrosequencing (PYR). After establishing the minimum PYR cut-off that could yield a significant …
Dental age estimation based on DNA methylation using real-time methylation-specific PCR
A Ogata, M Kondo, M Yoshikawa, M Okano… - Forensic Science …, 2022 - Elsevier
… We recently quantified the methylation levels of elongation of very long chain fatty acids
protein 2 (ELOVL2) in teeth using real-time methylation-specific polymerase chain reaction (RT-…
protein 2 (ELOVL2) in teeth using real-time methylation-specific polymerase chain reaction (RT-…
A newly developed age estimation method based on CpG methylation of teeth-derived DNA using real-time methylation-specific PCR
M Kondo, H Aboshi, M Yoshikawa, A Ogata… - Journal of Oral …, 2021 - jstage.jst.go.jp
… In this study, an age estimation method based on methylation levels of DNA extracted from
teeth using real-time methylation-specific PCR (MSP) was developed. The CpG island in the …
teeth using real-time methylation-specific PCR (MSP) was developed. The CpG island in the …
Quantitative methylation-specific PCR: a simple method for studying epigenetic modifications of cell-free DNA
L Sigalotti, A Covre, F Colizzi, E Fratta - Cell-free DNA as Diagnostic …, 2019 - Springer
… To date, several approaches for measuring cfDNA methylation have been established,
including quantitative methylation-specific PCR (qMSP), which represents a simple, fast, and cost-…
including quantitative methylation-specific PCR (qMSP), which represents a simple, fast, and cost-…
[HTML][HTML] Detection of non-metastatic non-small-cell lung cancer in urine by methylation-specific PCR analysis: A feasibility study
… methylation-specific PCR (qMSP). The accuracy for NSCLC detection was determined by
univariable and multivariable logistic regression analysis, followed by leave-one-out cross-…
univariable and multivariable logistic regression analysis, followed by leave-one-out cross-…
[HTML][HTML] Methylation Density Pattern of KEAP1 Gene in Lung Cancer Cell Lines Detected by Quantitative Methylation Specific PCR and Pyrosequencing
FP Fabrizio, A Sparaneo, F Centra… - International Journal of …, 2019 - mdpi.com
… The methylation status was assessed using quantitative methylation specific PCR (QMSP)
and pyrosequencing, and the performance of the two assays was compared. Results. …
and pyrosequencing, and the performance of the two assays was compared. Results. …
[HTML][HTML] Triage of hrHPV-positive women: comparison of two commercial methylation-specific PCR assays
C Dippmann, M Schmitz, K Wunsch, S Schütze… - Clinical …, 2020 - Springer
… The QIAsure Methylation Test (Qiagen, Hilden, Germany) analyses in a multiplex
methylation-specific real-time PCR the methylation of the promoter regions of FAM19A4 and …
methylation-specific real-time PCR the methylation of the promoter regions of FAM19A4 and …
Prediction of cervical cancer precursor lesions by quantitative methylation specific PCR: a retrospective study
M Al Roomy, W Chehadeh, R Al Awadhi - Cytopathology, 2023 - Wiley Online Library
… HPV DNA was detected by real-time PCR, and HPV genotypes were identified by Sanger-…
-124-2 genes was detected by a quantitative methylation-specific PCR (qMSP) test using the …
-124-2 genes was detected by a quantitative methylation-specific PCR (qMSP) test using the …
[HTML][HTML] DNA methylation validation methods: a coherent review with practical comparison
Š Šestáková, C Šálek, H Remešová - Biological procedures online, 2019 - Springer
… assessment: methylation specific restriction endonucleases … methylation specific high-resolution
DNA melting (MS-HRM) and quantitative methylation specific polymerase chain reaction …
DNA melting (MS-HRM) and quantitative methylation specific polymerase chain reaction …
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