Multiplex genome editing by natural transformation in Vibrio mimicus with potential application in attenuated vaccine development
Z Yu, E Wang, Y Geng, K Wang, D Chen… - Fish & Shellfish …, 2019 - Elsevier
… Multiplex genome editing by natural transformation (MuGENT… DNA and insert it into genome
by natural transformation assay. … showed that combination of natural transformation and FLP-…
by natural transformation assay. … showed that combination of natural transformation and FLP-…
Expanding the capabilities of MuGENT for large-scale genetic engineering of the fastest-replicating species, Vibrio natriegens
LD Glasgo, KL Lukasiak, ER Zinser - Microbiology Spectrum, 2024 - Am Soc Microbiol
… genetic manipulation. Here, we expand on the capabilities of multiplex genome editing by
natural transformation (MuGENT) … utility as an emerging model organism for synthetic biology. …
natural transformation (MuGENT) … utility as an emerging model organism for synthetic biology. …
NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens
… for multiplex genome editing by natural transformation (MuGENT) 20 . Natural transformation
… integration of this DNA into the genome by RecA mediated homologous recombination 21 . …
… integration of this DNA into the genome by RecA mediated homologous recombination 21 . …
Establishing the fast-growing bacterium Vibrio natriegens as a next-generation chassis for synthetic biology
D Stukenberg - 2024 - archiv.ub.uni-marburg.de
… for multiplex genome editing by natural transformation (MuGENT)20. Natural transformation
… method combining the highly efficient genome editing through natural transformation with a …
… method combining the highly efficient genome editing through natural transformation with a …
Natural transformation in a classical-biotype Vibrio cholerae strain
CJ Lloyd, A Mejia-Santana, TN Dalia… - Applied and …, 2021 - Am Soc Microbiol
… This technique was also used to facilitate cotransformation into O395 of unmarked DNA (ΔlacZ,
ΔflaA, ΔflgG) for multiplex genome editing by natural transformation (MuGENT). These …
ΔflaA, ΔflgG) for multiplex genome editing by natural transformation (MuGENT). These …
Vibrio species as next-generation chassis for accelerated synthetic biology
C Hong, Y Kim, H Lee, S Yun, HG Lim, J Yang… - … Bioprocess Engineering, 2024 - Springer
… Additionally, multiplex genome editing by natural transformation (MuGENT) was
employed to engineer V. natriegens [17]. This involved replacing the native promoter of V. …
employed to engineer V. natriegens [17]. This involved replacing the native promoter of V. …
Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis
A Deng, Z Sun, T Wang, D Cui, L Li, S Liu… - Frontiers in …, 2021 - frontiersin.org
… led to the development of several molecular biology techniques for complete multiplex …
natural transformation, MuGENT was established to achieve multiplex genome editing in Vibrio …
natural transformation, MuGENT was established to achieve multiplex genome editing in Vibrio …
Efficient natural plasmid transformation of Vibrio natriegens enables zero-capital molecular biology
DA Specht, TJ Sheppard, F Kennedy, S Li… - PNAS …, 2024 - academic.oup.com
… (MuGENT), originally demonstrated in Vibrio cholerae, high … curiosity or just a tool for genomic
engineering but allows for … as a natural transformation-based host for molecular biology …
engineering but allows for … as a natural transformation-based host for molecular biology …
Synthetic Biology Tools for the Fast-Growing Marine Bacterium Vibrio natriegens
T Tschirhart, V Shukla, EE Kelly… - … synthetic biology, 2019 - ACS Publications
… in synthetic biology and metabolic engineering, our ability to meaningfully exploit biological
systems … In order to fully utilize a broader spectrum of natural and engineered capabilities, a …
systems … In order to fully utilize a broader spectrum of natural and engineered capabilities, a …
Vibrio natriegens: an ultrafast‐growing marine bacterium as emerging synthetic biology chassis
… engineering techniques, such as recombineering, multiplexed genome editing via natural
transformation (MuGENT) and the use of the CRISPR-Cas9 system. Please refer to the main …
transformation (MuGENT) and the use of the CRISPR-Cas9 system. Please refer to the main …