Bicelles rich in both sphingolipids and cholesterol and their use in studies of membrane proteins
… This gap is addressed by showing that sphingomyelin and cholesterol-rich (SCOR) lipid …
domain of the SCOR bicelles, indicating a higher proportion of trans conformations for the acyl …
domain of the SCOR bicelles, indicating a higher proportion of trans conformations for the acyl …
[HTML][HTML] Cholesterol-induced conformational change in the sphingomyelin headgroup
S Hanashima, K Murakami, M Yura, Y Yano… - Biophysical …, 2019 - cell.com
… In this study, we aimed to elucidate the Cho effect on the conformation and orientation of
the SM headgroup in SSM unitary and SSM/Cho (1:1) membranes by solid-state NMR and …
the SM headgroup in SSM unitary and SSM/Cho (1:1) membranes by solid-state NMR and …
Beyond detergent micelles: The advantages and applications of non-micellar and lipid-based membrane mimetics for solution-state NMR
K Klöpfer, F Hagn - Progress in nuclear magnetic resonance spectroscopy, 2019 - Elsevier
… are relevant and suitable for NMR-based investigations on the structure … The morphology
of bicelles depends on the total … SapA is a sphingolipid activator protein in lysosomes at a pH …
of bicelles depends on the total … SapA is a sphingolipid activator protein in lysosomes at a pH …
Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: Sphingomyelin clusters forming nano-subdomains are a characteristic …
… paper, gel-state subdomains are formed by sphingomyelin (SM) or dhSM, together with …
conformation around the SM amide group was examined using solution NMR with small bicelles, …
conformation around the SM amide group was examined using solution NMR with small bicelles, …
Evaluation of molecular ordering in bicelle bilayer membranes based on induced circular dichroism spectra
K Suga, K Kitagawa, S Taguchi, Y Okamoto… - Langmuir, 2020 - ACS Publications
… Sphingolipids are important biological membrane components, especially in lipid rafts,
and sphingolipid bicelle are advantageous for NMR membrane protein analysis. (59) Bovine …
and sphingolipid bicelle are advantageous for NMR membrane protein analysis. (59) Bovine …
[HTML][HTML] Theonellamide A, a marine-sponge-derived bicyclic peptide, binds to cholesterol in aqueous DMSO: Solution NMR-based analysis of peptide-sterol …
K Cornelio, RA Espiritu, S Hanashima… - … et Biophysica Acta (BBA …, 2019 - Elsevier
… Micelles do not incorporate Chol, and bicelles contain only 10 mol% of Chol, which does
not … Among other possible conformational models, we selected this structure because it was …
not … Among other possible conformational models, we selected this structure because it was …
[HTML][HTML] Membrane protein structure determination and characterisation by solution and solid-state nmr
V Yeh, A Goode, BB Bonev - Biology, 2020 - mdpi.com
… to study membrane protein folding, interactions, conformational … conversion between a
micellar and a bicellar phase [28]. … incorporation of sterols, sphingomyelin, gangliosides or other …
micellar and a bicellar phase [28]. … incorporation of sterols, sphingomyelin, gangliosides or other …
[HTML][HTML] Structure and dynamics of GPCRs in lipid membranes: physical principles and experimental approaches
… analysis led to the proposal of a putative sphingolipid binding … the use of bicelles in
solution-state NMR-based GPCR studies … to reveal an ever-richer conformational energy …
solution-state NMR-based GPCR studies … to reveal an ever-richer conformational energy …
Structure and dynamics of influenza M2 proton channels from solid-state NMR
VS Mandala - 2021 - dspace.mit.edu
… and oriented bicelles both show the apo protein undergoes conformational exchange at a rate
of … Conformational analysis of the full-length M2 protein of the influenza A virus using solid-…
of … Conformational analysis of the full-length M2 protein of the influenza A virus using solid-…
[HTML][HTML] G protein-coupled receptor (GPCR) reconstitution and labeling for solution nuclear magnetic resonance (NMR) studies of the structural basis of …
… -like complexes formed by the sphingolipid-activating protein and … bicelles to immobilize
and align the protein for solid-state NMR experiments. However, when using detergent bicelles …
and align the protein for solid-state NMR experiments. However, when using detergent bicelles …