… Similarly, RT is used to denote real-time PCR rather than reverse transcription, thus causing
confusion over which method is being described. For this Beginner’s Guide, we will be …

This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …

… PCR assays could be cumbersome and slow, continued advances in methods and technologies
used for the detection of PCR … of PCR amplification by fluorescence (real-time PCR) …

… Digital PCR (dPCR) has developed considerably since the publication of the … PCR
Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, …

… Here we have addressed several limitations of the ARTIC multiplex PCR method. To
eliminate systematic dropouts we introduced the V3 primer scheme which showed the highest …

… , the choice between PCR-RFLP and PCR-SSCP is largely … a useful guide for comparing
PCR-RFLP and PCR-SSCP in … to the practical applications of both techniques in terms of …

… through restriction digestion, PCR or molecular barcoding. These later methods do not require
upfront … The ease of use of some of these methods has spearheaded their application in …

… PCR cycle number separately for each sample based on the amplification plots obtained from
the qPCR measurement (see Box 2 for instructions). … all reactions reach PCR plateau (see …

Antibodies are a key resource in biomedical research yet there are no community-accepted
standards to rigorously characterize their quality. Here we develop a procedure to validate …

… guidelines are referred to as procedure standards. Any previous … guideline or procedure
guideline that describes how to perform a procedure is now considered an SNMMI procedure …