… bottlenecks: expression of complementary enzymes and permeabilization of cell membranes to improve performance of CYP153A6 in Escherichia coli whole cell …

BE White - 2022 - open.uct.ac.za
… sites”; lack of oxygen for biotransformation and cell metabolism; poor transport of n-octane …
biotransformation rates or product titres in biotransformations carried out with whole cells. …

[HTML][HTML] Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical …

Y Wang, S Sun, L Yu, S Hu, W Fan, F Leng… - Ultrasonics Sonochemistry, 2021 - Elsevier
… The cetyl trimethyl ammonium bromide (CTAB) was also … , which depends on the growth
and metabolism process of E. … of temperature on the outer membrane permeabilization of E. …

[HTML][HTML] L-Carnitine Production Through Biosensor-Guided Construction of the Neurospora crassa Biosynthesis Pathway in Escherichia coli

P Kugler, M Trumm, M Frese… - … in Bioengineering and …, 2021 - frontiersin.org
… of L-carnitine out of the E. coli cell may also limit biotransformation and its de novo production.
… exchange of intracellular and extracellular trimethylammonium compounds such as L-…

[HTML][HTML] Multi-Omic Analysis to Characterize Metabolic Adaptation of the E. coli Lipidome in Response to Environmental Stress

T Kralj, M Nuske, V Hofferek, MA Sani, TH Lee… - Metabolites, 2022 - mdpi.com
… fatty acid biosynthesis and cell wall metabolism, decreased PG, … have also been reported
for Gram-negative Escherichia coli (E. … coli to membrane permeabilization by the antimicrobial …

[PDF][PDF] Development of sustainable platforms for the stabilization and purification of the recombinant Green Fluorescent Protein produced by Escherichia coli

NV DOS SANTOS - repositorio.unesp.br
… estabilidade das biomoléculas, aliados ao desenvolvimento de plataformas mais simples,
baratas e sustentáveis para estabilização e purificação … path length of the CD cell in mm p(r) …

Integrated gene engineering synergistically improved substrate-product transport, cofactor generation and gene translation for cadaverine biosynthesis in E. coli

T Osire, T Yang, M Xu, X Zhang, M Long… - International Journal of …, 2021 - Elsevier
… cadaverine transport in and out of the cell membrane, respectively. Additionally, pyridoxal
… of cellular reactions and is a requirement for decarboxylase whole-cell biotransformation [2]. …

Chitosan quaternary ammonium salt induced mitochondrial membrane permeability transition pore opening study in a spectroscopic perspective

C Xia, B Fu, X Zhang, C Qin, JC Jin - International Journal of Biological …, 2020 - Elsevier
… activities of quaternized chitosan against Escherichia coli (E. … in regulating cellular metabolism
and coordinating cell death … on mitochondrial inner membrane permeabilization to H + , …

[HTML][HTML] Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli

S Gao, D Ma, Y Wang, A Zhang, X Wang, K Chen - BMC biotechnology, 2023 - Springer
… In this study, whole-cell bioconversion was … bioconversion of dopamine by whole-cell
catalysis. This research provided reference for whole-cell catalysis which is hindered by cell

The production of enantiopure D-lysine from L-lysine by a two-strain coupled system

S Xu, X Lu, X Wang, Z Gao, K Chen… - Chinese Journal of …, 2020 - Elsevier
… reported in recombinant Escherichia coli overexpressing 5-… 80 and betaine for increased
cell permeabilization, could also … In this work, a two-strain coupling whole-cell bioconversion

[HTML][HTML] Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol

KN Schwaiger, M Cserjan-Puschmann… - Microbial Cell …, 2021 - Springer
… Building on Escherichia coli as host for recombinant production of SucP, the analogous …
In summary, freeze–thaw treatment appears to be suitable for E. coli cell permeabilization. It …