Background: Human Papillomavirus (HPV) infection has been incriminated in cervical cancer. Twenty of more than 100 types were classified as highrisk (HR), and associated with cervical cancer and precancerous lesions. The great question that this article wants to answer is, is there any new genome responsible for the cervical cytological changes other than the known high risk human papilloma virus gene. The second aim of this study is to raise awareness about HPV and cervical cancer and to draw attention to the need for more studies about this subject.

Materials and method: A cross sectional study with a convenience sample of 188 females was used. Information was obtained by interviewing the patients. Pap smear was done for all patients. HPV screening, and genotyping test was done for 151 patients.

Results: Positive HPV Pap smear (Positive HPV Pap) was found in 31 (16.5%) patients, while (Positive HPV test) was found among 4 cases (2.7%). Negative HPV test/Positive HPV Pap was found among 31 (20.5%). Three HPV genes were detected HPV (16, 18, 65), 2 (50%), 1 (25%), 1 (25%), respectively. The mean age for those who had Negative HPV test/Positive HPV Pap (33.5±8.3), was significantly lower than those had Positive HPV test/Negative HPV Pap (38.8±11.1), and those (Negative HPV/Positive HPV Pap) was found among 31 (20.5%). Three HPV genes were detected HPV 16, 18, 65, 2 (50%), 1 (25%), 1 (25%), respectively. The mean age for those who had (Negative HPV/Positive HPV Pap)(33.5±8.3), was significantly lower than those who had Positive HPV/Negative HPV Pap (38.8±11.1), and those with (Negative HPV/Negative Pap)(41.2±118).

Conclusion: Heterogeneity was common among Iraqi patients, decreasing with increasing age. Results suggest presence of a new genotype.