… in enzyme engineering, it has become possible to improve catalytic … (h) Inverting GPs promote
synthesis via either axial-to-… Most glycosidases used for synthetic purposes have an exo-…

… from rationally modifying specific catalytic residues, over targeted … to promote different types
of HMO synthesis reactions. The … were obtained with engineered glycosidases compared to …

… engineered glycosidases or glycosyl hydrolases (GHs) offer a promising avenue for the
synthesis of glycans like oligosaccharides … results of federally sponsored research in accordance …

… makes glycosidases and phosphorylases applicable to the … The active site of ENGases
contains two catalytic residues: a … (75i) With the emergence of new tools of genetic engineering, …

… of either the catalytic base residue of inverting glycosidases or the nucleophile of retaining
… the protein engineering methods and reaction condition control to promote the fucosidase-…

… CAZyme substrate and catalytic promiscuity by reactions as … was recently engineered to
produce fructo-oligosaccharides from sucrose … in combination with strain-promoted azide-alkyne …

… and a non-catalytic loop domain that promoted acceptor sugar … engineered to glycosynthases
by mutating their catalytic … in glycosyl transferase and modified glycosidase reactions. The …

… pathogenic infections and promoting the development of … Glycosynthases, an engineered
mutant glycosidase, have … low catalytic activity for lactose in the large-scale synthesis of 3…

… but also bioactive nutrients for promoting the development of the … Some glycosidases, which
possess trans-glycosidase or … The catalytic reaction by GTs is the last and limiting step for …

… in synthetic biology and metabolic engineering techniques … effects and improving the
catalytic efficiency of the enzyme. … Significant improvements in our understanding of the catalytic …