Clostridioides difficile is a Gram-positive bacillus, which is a frequent cause of
gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the
frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile
persistence and recurrence, including biofilm formation, are of increasing interest.
Previously, our group and others found that type IV pili, filamentous helical appendages
polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile …