Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida

DC Volke, J Turlin, V Mol, PI Nikel - Microbial Biotechnology, 2020 - Wiley Online Library
… /promoter pair from the soil bacterium Pseudomonas putida … in monocopy ensures low
transcription levels of the regulator … and activates transcription from the Pm promoter.D. The trans…

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

J Kim, A Goñi-Moreno, V de Lorenzo - Mbio, 2021 - Am Soc Microbiol
controls expression of the xyl genes and their high-level interplay with the host’s physiological
… complete replacement of the native σ 54 -dependent Pu promoter of the upper operon of …

Narrative of a versatile and adept species Pseudomonas putida

M Kivisaar - Journal of medical microbiology, 2020 - microbiologyresearch.org
… is driven by the promoter Pu, which is recognized by the alternative σ factor, σ 54 , and is
activated … metabolic modules, deeper understanding of physiology and energy metabolism of P. …

Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization in Pseudomonas putida M2

S Shrestha, D Awasthi, Y Chen, J Gin… - Applied and …, 2023 - Am Soc Microbiol
… glucose inhibited TOL plasmid Pu promoter for toluene catabolism (… (28, 54), to identify sRNAs
involved in CCR in Pseudomonas … were loaded onto an Ascentis (Sigma–Aldrich) ES-C18 …

[HTML][HTML] The regulatory hierarchy following signal integration by the CbrAB two-component system: diversity of responses and functions

E Monteagudo-Cascales, E Santero, I Canosa - Genes, 2022 - mdpi.com
… signal detection have also been described [54,55]. In all cases, … putida, the P crcZ and
P crcY promoters (and P crcZ in P. … , including ECF sigma factors, as well as post-transcriptional …

[HTML][HTML] How Do Bacteria Maximize Their Cellular Assets?

J Kim - 2021 - mbl.or.kr
… by the plasmid pWW0 of Pseudomonas putida mt-2. This soil … system, activates both the Pu
promoter (responsible for the … can be controlled by the intracellular alarmone [54−58]. When …

Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells

XJ Chen, B Wang, IP Thompson… - ACS Synthetic …, 2021 - ACS Publications
… ) binding sites of the P norV promoter, we demonstrated that the … The P norV promoter is
a σ (54)-dependent promoter, … from M9 5x minimal salts (Sigma-Aldrich) supplemented with …

[PDF][PDF] Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-cis, cis-muconate in engineered Pseudomonas putida

NT Wirth, PI Nikel - Chem Catalysis, 2021 - cell.com
… versatile metabolism and high levels of stress resistance.Inspired by … control was exerted
by the Pm, Pben, or Pcat promoters … A similar effect of physiological control mechanisms was …

[HTML][HTML] Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

…, C Pan, X Li, N Hao, T Zhang, MJ Gaffrey, Y Pu… - Biotechnology for …, 2021 - Springer
… [20], the optimization of promoter or the ribosome binding site (… putida KT2440 under
demanding physiological conditions. … /NADP + maintained at low level until 48 h of fermentation …

Noise in bacterial gene expression

C Engl - Biochemical Society Transactions, 2019 - portlandpress.com
levels of noise, implying a selective pressure for population-wide homogenous expression
of genes with fundamental roles in cell physiology. … on sigma5454 )-dependent promoters