Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

GM Baci, AA Cucu, AI Giurgiu, AS Muscă, L Bagameri… - Insects, 2021 - mdpi.com
… The life sciences research fields were revolutionized by the outstanding development of
various genome editing tools. By using specific techniques of genome editing, the genomic DNA …

Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericulture industry

SY Ma, G Smagghe, QY Xia - Insect science, 2019 - Wiley Online Library
… In recent years, research in life sciences has been remarkably revolutionized owing to the
establishment, development and application of genome editing technologies. Genome editing …

Development of SpyTag/SpyCatcher-bacmid expression vector system (SpyBEVS) for protein bioconjugations inside of silkworms

J Xu, T Kato, EY Park - International Journal of Molecular Sciences, 2019 - mdpi.com
… into the silkworm-bacmid protein expression system (SpyBEVS… in cultured silkworm cells
and in vivo in silkworm larvae by co-… Given the flexibility and robustness of silkworm-BEVS, our …

[PDF][PDF] Study on the efficient purification of recombinant proteins from the silkworm expression system

R Minkner - 2019 - shizuoka.repo.nii.ac.jp
… So prefer young silkworms 28C by a relative humidity of 80 %, but older silkworms 24C by …
-PAGE gels the different protein expression patterns of the silkworm strains Nistari, P2D 1 and …

Silkworm Transgenesis and its Applications

H Sezutsu, T Tamura - Transgenic Insects: Techniques and …, 2022 - books.google.com
… First, the expression of the transgene in the silkworm is weaker than that of the … expression
system enhances its activity. Secondly, it facilitates the generation of transgenic silkworms, …

[PDF][PDF] Transgenic Bombyx mori as a biotechnological platform to produce recombinant proteins.

GM Baci, AR Moise, DS Dezmirean - Scientific Papers. Series D …, 2021 - researchgate.net
… We herein, by highlighting the pivotal role of Bombyx mori in life sciences, review the …
Silkworm expression system as a platform technology in life science. Applied Microbiology …

TALEN-mediated homologous-recombination-based fibroin light chain in-fusion expression system in Bombyx mori

S Yu, H Zheng, X Ye, X Dai, X Wang, S Zhao… - … in Bioengineering and …, 2024 - frontiersin.org
… FibL expression system in silkwormexpression system by TALEN-mediated HDR in silkworm,
and shown that an exogenous gene could be expressed at satisfactory levels in this system

Active human and murine tumor necrosis factor α cytokines produced from silkworm baculovirus expression system

T Ebihara, J Xu, Y Tonooka, T Nagasato, K Kakino… - Insects, 2021 - mdpi.com
… TNFα proteins are successfully expressed in the baculovirus expression system using
silkworm larvae as hosts. The biological activities of purified products were verified in culture …

Innovations in the insect cell expression system for industrial recombinant vaccine antigen production

MMJ Cox - Vaccines, 2021 - mdpi.com
… This review summarizes recent innovations in technology areas important for enhancing
recombinant protein production levels using the baculovirus–insect cell expression system and …

Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines

W Lu, R Wang, P Wang, S Ma, Q Xia - Insects, 2021 - mdpi.com
… The silkworm genetic code expansion system established here is another useful
controllable gene expression system besides tetracycline induced expression system. …